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AB5350-200UL | Anti-Potassium Channel SK3 Antibody

AB5350-200UL
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5350-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel SK3 Antibody
      Alternate Names
      • KCa3
      • Kcnn3
      • SKCa3
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
      Applications
      ApplicationAnti-Potassium Channel SK3 Antibody detects level of Potassium Channel SK3 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blotting: 1.0-1.5 μg/mL (1:200-1:300)

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHighly purified peptide corresponding to residues 2-21 of human SK3 (Accession Q9UGI6).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes SK3. The epitope specific for SK3 is not present in any other known proteins. The immunogen sequence is identical in mouse and porcine.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAction potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. This gene is a member of the KCNN family of potassium channel genes. Two transcript variants encoding two different isoforms have been found for this gene. One of the variants lacks the CAG repeat regions.
      Gene Symbol
      • KCNN3
      • OTTHUMP00000042458
      • OTTHUMP00000042459
      • SKCA3
      • hSK3
      • KCa2.3
      • SKCa3
      • K3
      • SK3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UGI6 # Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
      SIZE: 736 amino acids; 82026 Da
      SUBUNIT: Hetero-oligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: Q9UGI6 ## Belongs to the potassium channel KCNN family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2375577 2375577
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2426535 2426535
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2460960 2460960
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) -2530992 2530992
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) -2716594 2716594
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) -2717820 2717820
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) -2734776 2734776
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) -2782561 2782561
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2294221 2294221

      References

      Reference overviewPub Med ID
      Isolation of a novel potassium channel gene hSKCa3 containing a polymorphic CAG repeat: a candidate for schizophrenia and bipolar disorder?
      Chandy, K G, et al.
      Mol. Psychiatry, 3: 32-7 (1998) 1998

      Show Abstract
      9491810 9491810

      Data Sheet

      Title
      RABBIT ANTI-SK3 (KCa2.3, KCa3, Kcnn3, SKCa3) AFFINITY PURIFIED POLYCLONAL ANTIBODY