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AB5360-200UL | Anti-Potassium Channel Kv4.2 Antibody

AB5360-200UL
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5360-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel Kv4.2 Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
      Applications
      ApplicationThis Anti-Potassium Channel Kv4.2 Antibody is validated for use in WB for the detection of Potassium Channel Kv4.2.
      Key Applications
      • Western Blotting
      Application NotesWestern blotting: 1.0-1.5 μg/mL (1:200-1:300)

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHighly purified peptide corresponding to residues 454-469 of rat Kv4.2 (Accession Q63881).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Kv4.2. The epitope specific for Kv4.2 is not present in any other known proteins.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVoltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels.
      Gene Symbol
      • KCND2
      • Kv4.2
      • KV4.2
      • MGC119703
      • RK5
      • KIAA1044
      • MGC119702
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9NZV8 # Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
      SIZE: 630 amino acids; 70537 Da
      SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND3. Interacts with DPP6, DLG4 and FREQ (By similarity). Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Interacts with FLNA, FLNC and DPP10.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
      TISSUE SPECIFICITY: Highly expressed throughout the brain. Expression is very low or absent in other tissues.DOMAIN:SwissProt: Q9NZV8 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      PTM: Phosphorylated on serine and threonine residues (By similarity).
      SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily.
      Molecular Weight70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-Kv4.2 -2515383 2515383
      RABBIT ANTI-Kv4.2 -2574853 2574853
      RABBIT ANTI-Kv4.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2274246 2274246

      References

      Reference overviewPub Med ID
      Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel.
      Baldwin, T J, et al.
      Neuron, 7: 471-83 (1991) 1991

      1840649 1840649
      Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart.
      Roberds, S L and Tamkun, M M
      Proc. Natl. Acad. Sci. U.S.A., 88: 1798-802 (1991) 1991

      Show Abstract
      1705709 1705709

      Data Sheet

      Title
      RABBIT ANTI-Kv4.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies