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AB5178 | Anti-Potassium Channel Kv1.3 Antibody

AB5178
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5178
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel Kv1.3 Antibody
      References
      Product Information
      FormatPurified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 20 ng of protein per lane (minigel). For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      ApplicationDetect Potassium Channel Kv1.3 using this Anti-Potassium Channel Kv1.3 Antibody validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein and a C-terminal portion of human Kv1.3 protein (amino acids 471-523) (Accession P22001).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes a full length Kv1.3 protein. Possibly cross reacts with Kv1.1.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPotassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T-cell proliferation and activation. This gene appears to be intronless and it is clustered together with KCNA2 and KCNA10 genes on chromosome 1.
      Gene Symbol
      • KCNA3
      • HLK3
      • HGK5
      • KV1.3
      • HuKIII
      • HPCN3
      • Kv1.3
      • MK3
      • HUKIII
      • PCN3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22001 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
      SIZE: 523 amino acids; 58304 Da
      SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: P22001 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Potassium Channel Kv1.3 - 2435030 2435030
      Anti-Potassium Channel Kv1.3_2842039 2842039

      References

      Reference overviewPub Med ID
      Deletion of voltage-gated channel affects glomerular refinement and odorant receptor expression in the mouse olfactory system.
      K C Biju,David Ronald Marks,Thomas Gerald Mast,Debra Ann Fadool
      The Journal of comparative neurology 506 2008

      Show Abstract Full Text Article
      18022950 18022950

      Data Sheet

      Title
      RABBIT ANTI-Kv1.3 (Kcna3) AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Alternative Packsize

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies