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AB5202-200UL | Anti-Potassium Channel IsK Antibody

AB5202-200UL
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5202-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel IsK Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      ApplicationDetect Potassium Channel IsK using this Anti-Potassium Channel IsK Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:100-1:200 on rat heart membranes, 1:200-1:1,000 on IsK expression Sf9 cells using ECL.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein from the C-terminal portion of human IsK protein (amino acids 67-129) (Accession P15382).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes a full length IsK protein. Does not cross react with any other potassium channel antigens tested so far.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryKCNE1 together with KCNQ1 encode the two subunits of the K+ channel responsible for the delayed-rectifier K+ current in cardiac myocytes (referred to as IsK).
      Gene Symbol
      • KCNE1
      • minK
      • JLNS2
      • MGC33114
      • ISK
      • MinK
      • LQT5
      • JLNS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15382 # Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).
      SIZE: 129 amino acids; 14675 Da
      SUBUNIT: Associates with KCNQ1/KVLQT1 and KCNH2/HERG.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed in heart, lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells.
      PTM: Phosphorylation inhibits the potassium current (By similarity).
      DISEASE: "SwissProt: P15382 # Defects in KCNE1 are a cause of the autosomal recessive Jervell and Lange-Nielsen syndrome (JLNS) [MIM:220400]. JLNS comprises profound congenital sensorineural deafness associated with syncopal episodes. These are caused by ventricular tachyarrhythmia secondary to abnormal repolarization, manifested by a prolonged QT interval on the electrocardiogram. & Defects in KCNE1 are the cause of long QT syndrome type 5 (LQT5) [MIM:176261]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE1 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents."
      SIMILARITY: SwissProt: P15382 ## Belongs to the potassium channel KCNE family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications