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AB5200-200UL | Anti-Potassium Channel GIRK2 Antibody

AB5200-200UL
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IHC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5200-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel GIRK2 Antibody
      Alternate Names
      • G-protein Activated Inwardly Rectifying Potassium Channel
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot use 20 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      ApplicationAnti-Potassium Channel GIRK2 Antibody detects level of Potassium Channel GIRK2 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein from the C-terminal portion of mouse GIRK2 protein (amino acids 374-414) (Accession P48542).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes a full length Kir3.2 (GIRK2, G-protein Activated Inwardly Rectifying Potassium Channel 2). Does not cross react with any other potassium channel antigens tested so far.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPotassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and may be involved in the regulation of insulin secretion by glucose. It associates with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.
      Gene Symbol
      • KCNJ6
      • KATP-2
      • KCNJ7
      • MGC126596
      • BIR1
      • KATP2
      • hiGIRK2
      • KIR3.2
      • Kir3.2
      • GIRK2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48051 # This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
      SIZE: 423 amino acids; 48451 Da
      SUBUNIT: Associates with GIRK1 or GIRK4 to form a G-protein- activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
      SIMILARITY: SwissProt: P48051 ## Belongs to the inward rectifier-type potassium channel family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-Kir3.2 (GIRK2) -2557541 2557541
      RABBIT ANTI-Kir3.2 (GIRK2) -2576330 2576330
      RABBIT ANTI-Kir3.2 (GIRK2) -2586508 2586508
      RABBIT ANTI-Kir3.2 (GIRK2) -2792520 2792520
      RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2477831
      RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2197108 2197108
      RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2274244 2274244
      RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2287686 2287686

      Data Sheet

      Title
      RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY