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MAB1596 | Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

MAB1596
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, M, R ICC, IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1596
      Replaces04-1066
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
      Alternate Names
      • PSD-95
      Background InformationPSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain, rat brain tissue.
      PresentationPurified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.
      Applications
      ApplicationAnti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      Representative image from a previous lot.
      Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.

      Immunocytochemistry:
      12.5 µg/mL

      Western Blot:
      1-5 μg/mL (ECL) on rat brain lysate (20 μg).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant rat PSD-95.
      Clone6G6-1C9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Mouse
      • Rat
      Species Reactivity NoteRat, mouse and bovine. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • PSD-95
      Purification MethodSee datasheet for purification method.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P78352 # Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity).
      SIZE: 724 amino acids; 80495 Da
      SUBUNIT: Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, and BEGAIN (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK.
      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
      TISSUE SPECIFICITY: Brain.
      DOMAIN: SwissProt: P78352 The PDZ domain 3 mediates interaction with ADR1B. & The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
      PTM: Palmitoylation of isoform 1 is required for targeting to postsynaptic density (By similarity).
      SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain.
      Molecular Weight~100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in Immunocytochemistry.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Post Synaptic Density Protein 95 2462797
      Anti-Post Synaptic Density Protein 95, -2780630 2780630
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2161095 2161095
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2370101 2370101
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2426503 2426503
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 1978745 1978745
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2021197 2021197
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2057815 2057815
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2280812 2280812
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - 2492127 2492127
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - JC1641533 JC1641533
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - LV1532660 LV1532660
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - LV1581097 LV1581097
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - NG1734308 NG1734308
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - NG1738987 NG1738987
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - NG1820161 NG1820161
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - NG1858223 NG1858223
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - NG1900669 NG1900669
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2558428 2558428
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2594859 2594859
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2620623 2620623
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2653142 2653142
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2665058 2665058
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2698443 2698443
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 -2728377 2728377
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9, Monoclonal Antibody 2828625

      References | 30 Available | See All References

      Reference overviewApplicationPub Med ID
      STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs.
      Garcia-Alvarez, G; Lu, B; Yap, KA; Wong, LC; Thevathasan, JV; Lim, L; Ji, F; Tan, KW; Mancuso, JJ; Tang, W; Poon, SY; Augustine, GJ; Fivaz, M
      Molecular biology of the cell 26 1141-59 2015

      Show Abstract
      25609091 25609091
      The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation.
      Nakano-Kobayashi, A; Tai, Y; Nadif Kasri, N; Van Aelst, L
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 8665-71 2014

      Show Abstract
      24966368 24966368
      High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
      Nieland, TJ; Logan, DJ; Saulnier, J; Lam, D; Johnson, C; Root, DE; Carpenter, AE; Sabatini, BL
      PloS one 9 e91744 2014

      Show Abstract
      Immunofluorescence24633176 24633176
      Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats.
      Bailey, EM; Green, SH
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 13110-26 2014

      Show Abstract
      25253857 25253857
      Localization of diacylglycerol lipase alpha and monoacylglycerol lipase during postnatal development of the rat retina.
      Cécyre, B; Monette, M; Beudjekian, L; Casanova, C; Bouchard, JF
      Frontiers in neuroanatomy 8 150 2014

      Show Abstract
      25565975 25565975
      Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons.
      Galic, M; Tsai, FC; Collins, SR; Matis, M; Bandara, S; Meyer, T
      eLife 3 e03116 2014

      Show Abstract
      25498153 25498153
      Ribbon synapse plasticity in the cochleae of Guinea pigs after noise-induced silent damage.
      Shi, L; Liu, L; He, T; Guo, X; Yu, Z; Yin, S; Wang, J
      PloS one 8 e81566 2013

      Show Abstract
      24349090 24349090
      An in vitro model of developmental synaptogenesis using cocultures of human neural progenitors and cochlear explants.
      Nayagam, BA; Edge, AS; Needham, K; Hyakumura, T; Leung, J; Nayagam, DA; Dottori, M
      Stem cells and development 22 901-12 2013

      Show Abstract
      23078657 23078657
      Expression of voltage-gated calcium channel α(2)δ(4) subunits in the mouse and rat retina.
      De Sevilla Müller, LP; Liu, J; Solomon, A; Rodriguez, A; Brecha, NC
      The Journal of comparative neurology 521 2486-501 2013

      Show Abstract
      23296739 23296739
      Low density lipoprotein receptor-related protein 1 (LRP1) modulates N-methyl-D-aspartate (NMDA) receptor-dependent intracellular signaling and NMDA-induced regulation of postsynaptic protein complexes.
      Nakajima, C; Kulik, A; Frotscher, M; Herz, J; Schäfer, M; Bock, HH; May, P
      The Journal of biological chemistry 288 21909-23 2013

      Show Abstract
      23760271 23760271

      Data Sheet

      Title
      Anti-Post Synaptic Density Protein 95, clone 6G6-1C9 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies