MAB5324 Anti-Polysialic Acid-NCAM Antibody, clone 2-2B

50 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Vrt ICC, IHC, RIA, WB M Ascites Monoclonal Antibody
      Catalogue NumberMAB5324
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Polysialic Acid-NCAM Antibody, clone 2-2B
      Alternate Names
      • PSA-NCAM
      Background InformationPolysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues.
      The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle,sprout, and branch appropriately during axon path-finding.
      Product Information
      • Positive control tissue: Young Rat Hippocampus, human hippocampus.
      PresentationMouse monoclonal IgM in buffer containing 50% glycerol, no preservatives.
      ApplicationDetect Polysialic Acid-NCAM using this Anti-Polysialic Acid-NCAM Antibody, clone 2-2B validated for use in IC, IH, RIA & WB with more than 30 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Radioimmunoassay
      • Western Blotting
      Application NotesWestern Blotting: A 1:500-1:1,000 concentration of a previous lot was used in WB.

      Immunocyto/histochemistry: A 1:200-1:400 dilution of a previous lot of this antibody worked on live and fixed cells, tissue sections either frozen or fixed with any kind of fixative. (Rougon et al., 1986; Theodosis et al., 1991, 1999). It is not recommend that the antibody be used on paraffin embedded tissue since it gives variable results.

      Radioimmunoassay (RIA) :
      A previous lot of this antibody was used in RIA. (Figarella-Branger et al., 1996)

      Cell sorting and cell panning (Ben-Hur et al., 1998)

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenViable Meningococcus group B (strain 355).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with alpha 2-8 linked neuraminic acid (NeuAc-alpha 2-8) n with n >10. This polymer is usually termed polysialic acid (PSA). In vertebrates PSA is linked to neural cell adhesion molecule (NCAM, CD56), in bacteria it is associated with capsula of meningococcus strain group B.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Vertebrates
      Species Reactivity NoteThe monoclonal works well on all species expressing PSA-NCAM including mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCAM1
      • CD56
      • NCAM-140
      • NCAM
      • NCAM-120
      • MSK39
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13592 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.| P13591 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
      SIZE: 761 amino acids; 83770 Da
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
      DOMAIN: SwissProt: P13592
      SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. | P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight150-300 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Immunohistochemistry on hippocampus.

      Immunohistochemistry(paraffin) Analysis:
      PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons.
      Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Polysialic Acid-NCAM, clone 2-2B - 21379942137994
      Anti-Polysialic Acid-NCAM, clone 2-2B - 21384172138417
      Anti-Polysialic Acid-NCAM, clone 2-2B - 23820972382097
      Anti-Polysialic Acid-NCAM, clone 2-2B - 24265052426505
      Anti-Polysialic Acid-NCAM, clone 2-2B - 20241712024171
      Anti-Polysialic Acid-NCAM, -25247132524713
      Anti-Polysialic Acid-NCAM, -25562702556270
      Anti-Polysialic Acid-NCAM, -25781052578105
      Anti-Polysialic Acid-NCAM, -26260502626050
      Anti-Polysialic Acid-NCAM, -26496662649666


      Reference overviewApplicationSpeciesPub Med ID
      Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism.
      Lundgaard, I; Li, B; Xie, L; Kang, H; Sanggaard, S; Haswell, JD; Sun, W; Goldman, S; Blekot, S; Nielsen, M; Takano, T; Deane, R; Nedergaard, M
      Nature communications  6  6807  2015

      Show Abstract
      25904018 25904018
      Lactoferrin Promotes Early Neurodevelopment and Cognition in Postnatal Piglets by Upregulating the BDNF Signaling Pathway and Polysialylation.
      Chen, Y; Zheng, Z; Zhu, X; Shi, Y; Tian, D; Zhao, F; Liu, N; Hüppi, PS; Troy, FA; Wang, B
      Molecular neurobiology  52  256-69  2015

      Show Abstract
      25146846 25146846
      Tis21 is required for adult neurogenesis in the subventricular zone and for olfactory behavior regulating cyclins, BMP4, Hes1/5 and Ids.
      Farioli-Vecchioli, S; Ceccarelli, M; Saraulli, D; Micheli, L; Cannas, S; D'Alessandro, F; Scardigli, R; Leonardi, L; Cinà, I; Costanzi, M; Mattera, A; Cestari, V; Tirone, F
      Frontiers in cellular neuroscience  8  98  2014

      Show Abstract
      24744701 24744701
      Epigenetic regulation by chromatin activation mark H3K4me3 in primate progenitor cells within adult neurogenic niche.
      Sandstrom, RS; Foret, MR; Grow, DA; Haugen, E; Rhodes, CT; Cardona, AE; Phelix, CF; Wang, Y; Berger, MS; Lin, CH
      Scientific reports  4  5371  2014

      Show Abstract
      24947819 24947819
      Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
      Mallon, BS; Hamilton, RS; Kozhich, OA; Johnson, KR; Fann, YC; Rao, MS; Robey, PG
      Stem cell research  12  376-86  2014

      Show Abstract
      24374290 24374290
      Molecular targets of chromatin repressive mark H3K9me3 in primate progenitor cells within adult neurogenic niches.
      Foret, MR; Sandstrom, RS; Rhodes, CT; Wang, Y; Berger, MS; Lin, CH
      Frontiers in genetics  5  252  2014

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)25126093 25126093
      Morphology, distribution and phenotype of polycystin kidney disease 2-like 1-positive cerebrospinal fluid contacting neurons in the brainstem of adult mice.
      Orts-Del'Immagine, A; Kastner, A; Tillement, V; Tardivel, C; Trouslard, J; Wanaverbecq, N
      PloS one  9  e87748  2014

      Show Abstract
      24504595 24504595
      Titration of GLI3 repressor activity by sonic hedgehog signaling is critical for maintaining multiple adult neural stem cell and astrocyte functions.
      Petrova, R; Garcia, AD; Joyner, AL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  17490-505  2013

      Show Abstract
      24174682 24174682
      The hippocampus of the eastern rock sengi: cytoarchitecture, markers of neuronal function, principal cell numbers, and adult neurogenesis.
      Slomianka, L; Drenth, T; Cavegn, N; Menges, D; Lazic, SE; Phalanndwa, M; Chimimba, CT; Amrein, I
      Frontiers in neuroanatomy  7  34  2013

      Show Abstract
      24194702 24194702
      Alzheimer's disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric amyloid-β peptide injection in rats.
      Zussy, C; Brureau, A; Keller, E; Marchal, S; Blayo, C; Delair, B; Ixart, G; Maurice, T; Givalois, L
      PloS one  8  e53117  2013

      Show Abstract
      23301030 23301030

      Data Sheet

      Anti-Polysialic Acid-NCAM, clone 2-2B - Data Sheet

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