MABS1252 | Anti-Polycystin-1 Antibody, clone E4 (5F4D2)

MABS1252
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M IH(P), IP, WB, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS1252
      DescriptionAnti-Polycystin-1 Antibody, clone E4 (5F4D2)
      Alternate Names
      • Polycystin-1
      • Autosomal dominant polycystic kidney disease 1 protein
      Background InformationPolycystin-1 (UniProt P98161; also known as Autosomal dominant polycystic kidney disease 1 protein) is encoded by the PKD1 gene (Gene ID 5310) in human. The primary cilium is a microtubule-based mechano- and chemo-sensory organelle that coordinates an array of cellular pathways during development and in tissue homeostasis, including Hedgehog (Hh), PDGFRα and Wnt signaling. Defective ciliary function causes a variety of ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). Polycystin-1 (PC1) and polycystin-2 (PC2) are large transmembrane proteins co-localized to primary cilium, where they play an important role in calcium-based signalling. PC1 is an atypical adhesion G-protein-coupled receptor (aGPCR) with 11-transmemrbane domains. PC1 is cis-autoproteolytically cleaved at a juxtamembrane GPCR cleavage site (GPS; a.a. 3012-3061) in a developmental stage-dependent manner, where PC1 is largely uncleaved in early embryonic kidneys but becomes extensively cleaved after birth. GPS cleavage results in a heterodimeric PC1 form, in which the N-terminal fragment (NTF) remains non-covalently associated with the transmembrane C-terminal fragment (CTF). Transgenic mice expressing non-cleavable PC1 develop cystic kidney disease during the postnatal period.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Polycystin-1 Antibody, clone E4 (5F4D2) is an antibody against Polycystin-1 for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: An 1:50 dilution of this antibody from a representative lot detected polycystin-1 immunoreactivity in rat and mouse kidney tissue sections.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated polycystin-1 from mouse lung tissue lysates (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
      Western Blotting Analysis: A representative lot detected exogenously expressed polycystin-1 in lysates from HEK cells transfected with human or mouse Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
      Western Blotting Analysis: A representative lot detected the polycystin-1 (PC1) GPS domain cis-autocleaved N-terminal fragment (NTF) in lysates from HEK cells transfected with full-length Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
      Immunocytochemistry Analysis: A representative lot and an anti-Arl13b antibody co-stained the primary cilium of 4% formaldehyde-fixed, 0.1% Triton X-100-permeabilized murine embryonic fibrolasts (mMEF) and collecting duct (CD)-derived cells by dual fluorescence immunocytochemistry. No polycystin-1 immunoreactivity was detected in Pkd1-knockout mMEFs (Kim, H., et al. (2014). Nat. Commun. 5:5482).
      Biological Information
      ImmunogenFLAG-tagged recombinant human polycystin-1 C-type lectin domain.
      EpitopeC-type lectin domain.
      CloneE4 (5F4D2)
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone E4 (also known as clone 5F4D2) targets polycystin-1 extracellular C-type lectin domain and immunostained wild-type, but not Pkd1-knockout murine cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PKD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightCalculated molecular weights of full-length/GPS domain autocleaved N-terminal fragment: 460.3/325.0 kDa (isoform 1), 459.1/324.0 kDa (isoform 2), 460.2/325.0 kDa (isoform 3).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human kidney tissue.

      Immunohistochemistry Analysis: An 1:250 dilution of this antibody lot detected polycystin-1 immunoreactivity in human kidney tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies