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MABD89 | Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, FC M Purified Monoclonal Antibody
      Catalogue NumberMABD89
      DescriptionAnti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84
      Alternate Names
      • Podocalyxin-like protein I
      • GCTM-2 antigen
      • Gp200
      Background InformationPodocalyxin-like protein 1 (PODXL) is a transmembrane protein known to be heavily sialyated and sulfated that is localized on the apical surface of podocytes and on vascular endothelia. Podocalyxin protein is expressed on the surface of undifferentiated human embryonic stem cells. Originally, the protein was characterized as a marker for vasculature and podocytes, but expression has since been observed in platelets, hematopoietic precursor cells and mesothelial cells from the coelmic cavity lining. Studies have outlined two distinct and seemingly adverse roles for this protein; it acts as an anti-adhesion molecule in renal glomerulae podocytes, but functions as an adhesion molecule within vasculature on high endothelial venules. Podocalyxin expression has been implicated in the development of aggressive cancers, beta catenin signaling, angiogenisis, and both myeloid and acute lymphoblastic leukemias.
      Product Information
      • H9 human embryonic stem cells.
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS without preservatives.
      ApplicationAnti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesThis antibody was shown to induce cell death of undifferentiated, but not differentiated hESC, within 30 minutes of incubation (Choo, A., et al. (2008). Stem Cells 26:1454-1463). This antibody has been shown to work in Western Blot applications (Choo, A., et al. (2008). Stem Cells, 26:1454–1463).
      This antibody has been shown to work in Flow Cytometry applications (Choo, AB., et al. (2008). Stem Cells, 26:1454–1463).
      Biological Information
      ImmunogenHES-3 Human embryonic stem cell line
      Clonemab 84
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the extracellular domain of Podocalyxin-like protein 1.
      This antibody has been shown to select against undifferentiated human embryonic stem cells (hESC) and to be functionally cytotoxic against these undifferentiated hESC populations (Choo, A., et al., 2008).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PCLP
      • Gp200
      • PC
      • PCLP1
      Purification MethodPurified by Ion-Exchange Chromatography
      UniProt Number
      Molecular Weight55 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Podocalyxin-like protein I in H9 human embryonic stem cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Podocalyxin-like protein I 2478030
      Anti-Podocalyxin-like protein I (Cytotoxic), clone mAb 84 - Q2074680 Q2074680
      Anti-Podocalyxin-like protein I (Cytotoxic), clone mAb 84 -2535299 2535299
      Anti-Podocalyxin-like protein I -2657492 2657492


      Reference overviewApplicationSpeciesPub Med ID
      Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1.
      Choo, Andre B, et al.
      Stem Cells, 26: 1454-63 (2008) 2008

      Show Abstract
      Flow CytometryHuman18356574 18356574

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      Life Science Research > Antibodies and Assays > Primary Antibodies