A part of MilliporeSigma

MAB10402 | Anti-Plexin B1 Antibody, clone 19A7.1

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMAB10402
      DescriptionAnti-Plexin B1 Antibody, clone 19A7.1
      Alternate Names
      • plexin B1
      • plexin-B1
      • PLEXIN-B1
      • Semaphorin receptor SEP
      Background InformationPlexin B1 is a member of the plexin family of proteins that are receptors for semaphorins, repulsive axon guidance molecules. Plexin B1 binds activated Rac and binds Rho when clustered causing stress fiber development. Plexin B1 may play a part in follicular growth and steroidogenesis in mouse ovaries. It is believed to play a role in trophoblast-endometrium interactions by increasing adhesion properties. Furthermore, Plexin B is thought to effect mouse development and tumor induced angiogenesis although it is excessive and not strictly required. Plexin B1 has also been shown to inhibit ovarian cancer cell movement and invasion when silenced.
      Product Information
      • Rat brain tissue lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      ApplicationAnti-Plexin B1 Antibody, clone 19A7.1 detects level of Plexin B1 & has been published & validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:50 dilution from a representative lot detected Plexin B1 in normal human, normal mouse, and normal rat brain tissues. Weak staining was also observed at 1:250. A dilution between 1-50 and 1-250 would be optimal.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Plexin B1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SEP
      • plexin 5
      • PLXN5
      • PLXNB1
      Purification MethodIon Exchange
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.

      SUBUNIT STRUCTURE: Monomer, and heterodimer with PLXNB2 after proteolytic processing. Binds RAC1 that has been activated by GTP binding. Interaction with SEMA4D promotes binding of cytoplasmic ligands. Binds PLXNA1 (By similarity). Interacts with ARHGEF11, ARHGEF12, ERBB2, MET, MST1R, RRAS, RHOD, RND1, NRP1 and NRP2.

      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein

      Isoform 2: Secreted

      Isoform 3: Secreted

      TISSUE SPECIFICITY: Highly expressed in fetal kidney, and at slightly lower levels in fetal brain, lung and liver.

      PTM: Phosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding.

      Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding.

      SEQUENCE SIMILARITIES: Belongs to the plexin family.

      Contains 3 IPT/TIG domains.

      Contains 1 Sema domain.

      SEQUENCE CAUTION: The sequence BAA23703.2 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight ~230 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Plexin B1 on 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information