A part of MilliporeSigma

05-947 | Anti-Phosphotyrosine Antibody, clone PY20

1 mg  
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      Replacement Information
      Replacement Information05-947 is a recommended replacement for MAB3080

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt ELISA, IP, WB M Purified Monoclonal Antibody
      Catalogue Number05-947
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone PY20
      Background InformationTyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes.
      Product Information
      • EGF-stimulated A431 cell lysate.

        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      Presentation0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C.
      ApplicationAnti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins.

      Recommended by an independent laboratory.
      Biological Information
      ImmunogenPhosphotyrosine conjugated to carrier protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityTyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein A Purfied
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot with RIPA lysates EGF-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of recipt.

      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine, clone PY20 - 2120120 2120120
      Anti-Phosphotyrosine, clone PY20 - 2387518 2387518
      Anti-Phosphotyrosine, clone PY20 - 2433434 2433434
      Anti-Phosphotyrosine, clone PY20 - 1957271 1957271
      Anti-Phosphotyrosine, clone PY20 - 1990769 1990769
      Anti-Phosphotyrosine, clone PY20 - 2200927 2200927
      Anti-Phosphotyrosine, clone PY20 - 2309056 2309056
      Anti-Phosphotyrosine, clone PY20 - 32559 32559
      Anti-Phosphotyrosine, clone PY20 - DAM1404746 DAM1404746
      Anti-Phosphotyrosine, clone PY20 - DAM1513990 DAM1513990
      Anti-Phosphotyrosine, clone PY20 - DAM1658693 DAM1658693
      Anti-Phosphotyrosine, clone PY20 - DAM1724030 DAM1724030
      Anti-Phosphotyrosine, clone PY20 - DAM1764405 DAM1764405
      Anti-Phosphotyrosine, clone PY20 - DAM1831394 DAM1831394
      Anti-Phosphotyrosine, clone PY20 - JBC1378224 JBC1378224
      Anti-Phosphotyrosine, clone PY20 - JBC1870061 JBC1870061
      Anti-Phosphotyrosine, clone PY20 - JBC1908435 JBC1908435
      Anti-Phosphotyrosine, clone PY20 - JBC1938964 JBC1938964
      Anti-Phosphotyrosine, clone PY20 -2517828 2517828
      Anti-Phosphotyrosine, clone PY20 -2604784 2604784
      Anti-Phosphotyrosine, clone PY20 -2607240 2607240
      Anti-Phosphotyrosine, clone PY20 -2775502 2775502
      Anti-Phosphotyrosine, clone PY20 -2779452 2779452


      Reference overviewPub Med ID
      Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells.
      Amantini, C; Morelli, MB; Santoni, M; Soriani, A; Cardinali, C; Farfariello, V; Eleuteri, AM; Bonfili, L; Mozzicafreddo, M; Nabissi, M; Cascinu, S; Santoni, G
      Oncoscience 2 395-409 2015

      Show Abstract
      26097873 26097873
      Rho-ROCK signaling differentially regulates chondrocyte spreading on fibronectin and bone sialoprotein.
      Gill, KS; Beier, F; Goldberg, HA
      American journal of physiology. Cell physiology 295 C38-49 2008

      Show Abstract Full Text Article
      18463228 18463228
      Protein tyrosine phosphatases and signalling.
      Stoker, Andrew W
      J. Endocrinol., 185: 19-33 (2005) 2005

      Show Abstract
      15817824 15817824
      Anchorage-dependent cell growth: tyrosine kinases and phosphatases meet redox regulation.
      Chiarugi, Paola and Giannoni, Elisa
      Antioxid. Redox Signal., 7: 578-92 (2005) 2005

      Show Abstract
      15890002 15890002
      Inhibitors of protein tyrosine phosphatases: next-generation drugs?
      Bialy, Laurent and Waldmann, Herbert
      Angew. Chem. Int. Ed. Engl., 44: 3814-39 (2005) 2005

      15900534 15900534
      Protein phosphorylation in signaling--50 years and counting.
      Pawson, Tony and Scott, John D
      Trends Biochem. Sci., 30: 286-90 (2005) 2005

      15950870 15950870
      Monoclonal antibodies to phosphotyrosine.
      Glenney, J R, et al.
      J. Immunol. Methods, 109: 277-85 (1988) 1988

      Show Abstract
      2452204 2452204

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