A part of MilliporeSigma

07-1473 | Anti-Phosphothreonine-Proline Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-1473
      DescriptionAnti-Phosphothreonine-Proline Antibody
      Background InformationPhosphothreonine-Proline, along with similar motifs involving serine/threonine residues preceding proline undergoing phosphorylation, has critical roles in the moderation of various cellular processes, protein function, and kinases through proline-directed phosphorylation. The deregulation of proline-directed phosphorylation is known to be a factor in diseases such as cancer and Alzheimer’s neurodegeneration.
      Product Information
      FormatAffinity Purified
      • Calyculin treated Jurkat cell lysate
      PresentationLiquid in TBS with 0.05% sodium azide and 50% glycerol.
      ApplicationDetect Phosphothreonine-Proline using this Anti-Phosphothreonine-Proline Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-phosphothreonine-proline amide
      (pT-P-NH2) peptide conjugates
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes the phosphorylated phothreonine-proline motif, which is the site of phosphorylation of many proline-directed protein kinase such MAPK and cyclin-dependent protein kinases. Also recognizes the phosphoserine-proline motif to a similar degree.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Calyculin treated Jurkat cell lysate.
      Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated proteins on 10 μg of calyculin treated Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information