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FCMAB106A4 | Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 Alexa Fluor® 488

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R FC, WB M AlexaFluor®488 Monoclonal Antibody
      Catalogue NumberFCMAB106A4
      DescriptionAnti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 Alexa Fluor® 488
      OverviewN-terminal c-Myc, GST-tagged, recombinant human Cardiac Troponin I full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
      Alternate Names
      • Transcription factor ISGF-3 components p91/p84
        signal transducer and activator of transcription 1
      Background InformationSTATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.
      Product Information
      • U937 cells
      PresentationPurified mouse monoclonal IgG1κ conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationAnti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 Alexa Fluor 488 is an antibody against Phospho-Stat1 (Tyr701) for use in FC & WB.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.
      EpitopePhosphorylated Tyr701
      SpecificityAntibody recognizes STAT1 phosphorylated at Tyr701.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
      Gene Symbol
      • ISGF-3
      • STAT-1
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFunction: Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
      Subunit structure: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter By similarity. Interaction with HCV core protein results in STAT1 degradation.
      Subcellular location: Cytoplasm. Nucleus. Note= Translocated into the nucleus upon activation by IFN-alpha/beta.
      PTM:Phosphorylated on tyrosine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Serine phosphorylation is also required for maximal transcriptional activity in IFN-gamma transduction (lacking in beta form).
      Involvement in disease: Defects in STAT1 are the cause of STAT1 deficiency [MIM:600555]. Patients generally suffer from mycobacterial or viral diseases. In the case of complete deficiency, patients can die of viral disease.
      Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
      Sequence similarities: Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
      ... hide » see more »
      Molecular Weight 88-92 kDa Calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry with U937 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 conjugate - NRG1677933 NRG1677933
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - 2118893 2118893
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - 2161171 2161171
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - 2005941 2005941
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - 2266782 2266782
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - NG1741012 NG1741012
      Milli-Mark Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor#174; 488 - NG1920072 NG1920072
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 - 2539998 2539998
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 -2610662 2610662
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 -2628297 2628297
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 -2660645 26604645
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 -2664434 2664434
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 -2719916 2719916
      Milli-Mark™ Anti-Phospho-Stat1 -2791199 2791199
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 - 2381745 2381745
      Milli-Mark™ Anti-Phospho-Stat1 (Tyr701), clone 5C9.2 Alexa Fluor® 488 - 2454698 2454698


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