16-222 Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate

16-222
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IF M FITC Monoclonal Antibody
      Description
      Catalogue Number16-222
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate
      Alternate Names
      • H3 histone, family 3A
      • H3S10P
      • Histone H3 (phospho S10)
      • H3 histone, family 3A
      • H3 histone, family 3B
      • H3 histone, family 3B (H3.3B)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatFITC
      Control
      • Mitotic HeLa cells (IF).
      PresentationPurified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.
      Applications
      ApplicationAnti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesWestern Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.
      Biological Information
      ImmunogenA proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.
      EpitopeSer10
      Cloneclone 3H10
      HostMouse
      SpecificityRecognizes histone H3 phosphorylated at Ser10, MW 17 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate2477929
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 21185112118511
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 23280202328020
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 23609482360948
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 22808632280863
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 3043630436
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - 32168753216875
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - DAM1479581DAM1479581
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - DAM1658675DAM1658675
      Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate - DAM1765615DAM1765615

      References

      Reference overviewPub Med ID
      Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice.
      Keqin Ren,Jing Lu,Aleksey Porollo,Chunying Du
      The Journal of biological chemistry  287  2012

      Show Abstract
      22396545 22396545
      Intercellular variation in signaling through the TGF-β pathway and its relation to cell density and cell cycle phase.
      Zieba, A; Pardali, K; Söderberg, O; Lindbom, L; Nyström, E; Moustakas, A; Heldin, CH; Landegren, U
      Molecular & cellular proteomics : MCP  11  M111.013482  2012

      Show Abstract
      22442258 22442258

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      Life Science Research > Antibodies and Assays > Primary Antibodies