A part of MilliporeSigma

04-1140 | Anti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, IH(P), ICC Rb Unpurified Monoclonal Antibody
      Catalogue Number04-1140
      DescriptionAnti-PTP1B Antibody, clone EP1841Y, rabbit monoclonal
      Alternate Names
      • Protein-tyrosine phosphatase 1B
      • protein tyrosine phosphatase 1B
      • protein tyrosine phosphatase, non-receptor type 1
      • protein tyrosine phosphatase, placental
      Background InformationPTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and is a drug discovery target in the fields of diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
      Product Information
      • HeLa, HepG2, and Jurkat cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PTP1B Antibody, clone EP1841Y, Rabbit is an antibody against PTP1B for use in WB, FC, IH(P) & IC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected PTP1B in human tonsil tissue.
      Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
      Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding Ser386 of human PTP1B.
      SpecificityThis antibody detects both phosphorylated and nonphosphorylated PTP1B.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • EC
      • OTTHUMP00000031266
      • PTP-1B
      • PTP1B
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades By similarity.
      CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight~50 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HeLa, HepG2, and Jurkat cell lysates.
      Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect PTP1B in HeLa, HepG2, and Jurkat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies