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CP35 | Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

CP35
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      CP35-100UL
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
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          Will advise
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the ~95 kDa PSD95 protein.
          Catalogue NumberCP35
          Brand Family Calbiochem®
          Application Data
          Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
          References
          ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
          Hualing, D., et al. 1997. Nature 386, 279.
          Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
          Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
          Cho., K.-O., et al. 1992. Neuron 9, 929.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (1:2000)
          Immunofluorescence (1:200, indirect)
          Immunoprecipitation (see comments)
          Application CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, rat PSD95
          ImmunogenRat
          Clone7E3-1B8
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          CP35

          References

          Reference overview
          Brakeman, P.R., et al. 1997. Nature 386, 284.
          Hualing, D., et al. 1997. Nature 386, 279.
          Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
          Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
          Cho., K.-O., et al. 1992. Neuron 9, 929.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-August-2007 RFH
          ApplicationImmunoblotting (1:2000)
          Immunofluorescence (1:200, indirect)
          Immunoprecipitation (see comments)
          Application Data
          Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.
          BackgroundEffective synaptic transmission requires the precise distribution and spatial localization of neurotransmitter receptors and ion channels on the postsynaptic nerve terminal. An understanding of synaptic development is derived largely from studies of the vertebrate skeletal neuromuscular junction. At glutamatergic synapses, the membrane associated cytoskeletal protein PSD-95 (Post Synaptic Density 95kDa) is believed to promote receptor clustering via interactions with the NR2 subunit of the NMDA receptor and the Shaker-type potassium channel. These interactions are likely mediated by multiple repeating PDZ domains (90 amino acid repeats). Two additional proteins, homer and GRIP, have recently been shown to interact with the metabotropic and AMPA receptors, respectively. It is likely that these proteins serve major functions in the molecular scaffolding and signal transduction process, which occurs at the synaptic junction.
          HostMouse
          Immunogen speciesRat
          Immunogenrecombinant, rat PSD95
          Clone7E3-1B8
          IsotypeIgG2a
          Speciesrat
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
          Hualing, D., et al. 1997. Nature 386, 279.
          Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
          Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
          Cho., K.-O., et al. 1992. Neuron 9, 929.
          Application referencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.