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05-1070 | Anti-PRAS40 Antibody, clone 9D10.2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R WB, IP, ICC M Purified Monoclonal Antibody
      Catalogue Number05-1070
      DescriptionAnti-PRAS40 Antibody, clone 9D10.2
      Alternate Names
      • 40 kDa proline-rich AKT substrate
      • AKT1 substrate 1 (proline-rich)
      • proline-rich Akt substrate, 40 kDa
      Background InformationPRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1.
      Product Information
      • NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      ApplicationThis Anti-PRAS40 Antibody, clone 9D10.2 is validated for use in WB, IP, IC for the detection of PRAS40.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.

      Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human PRAS40.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes PRAS40.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].
      Gene Symbol
      • AKT1S1
      • Lobe
      • PRAS40
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.

      SIZE: 256 amino acids; 27383 Da

      SUBUNIT: The phosphorylated form interacts with 14-3-3.

      SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).

      TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
      Molecular Weight~ 40 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PRAS40, clone 9D10.2 2485672
      Anti-PRAS40, clone 9D10.2 - 2026406 2026406
      Anti-PRAS40, clone 9D10.2 - 2197307 2197307
      Anti-PRAS40, clone 9D10.2 - NG1809746 NG1809746
      Anti-PRAS40, clone 9D10.2 - NG1882232 NG1882232
      Anti-PRAS40, clone 9D10.2 - NRG1713920 NRG1713920
      Anti-PRAS40, clone 9D10.2 -2750259 2750259


      Reference overviewPub Med ID
      Inhibition of HSP27 alone or in combination with pAKT inhibition as therapeutic approaches to target SPARC-induced glioma cell survival.
      Schultz, CR; Golembieski, WA; King, DA; Brown, SL; Brodie, C; Rempel, SA
      Molecular cancer 11 20 2012

      Show Abstract
      22480225 22480225

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      Life Science Research > Antibodies and Assays > Primary Antibodies