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07-1218 | Anti-PP1γ1 Antibody

07-1218
100 µg  
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      Overview

      Replacement Information
      Replacement Information07-1218 is a recommended replacement for AB4084

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, R WB, IP Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1218
      ReplacesAB4084
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP1γ1 Antibody
      Alternate Names
      • Protein phosphatase 1C catalytic subunit
      • Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
      • Protein phosphatase 1, catalytic subunit, gamma isoform
      • Serine/threonine phosphatase 1 gamma
      Background InformationType 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Cell Lysate
      PresentationPurified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
      Applications
      ApplicationAnti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.
      Biological Information
      ImmunogenThe immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPP1CC
      • PP-1G
      • PPP1G
      • EC 3.1.3.16
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36873 # Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.
      COFACTOR: Binds 1 iron ion per subunit. & Binds 1 manganese ion per subunit.
      SIZE: 323 amino acids; 36984 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with cyanobacterial toxin microcystin; disulfide-linked. Interacts with PPP1R7. Isoform gamma-2 interacts with SPZ1 (By similarity). Interacts with CDCA2.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with SPZ1 in the nucleus (By similarity).
      SIMILARITY: SwissProt: P36873 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      MISCELLANEOUS: Microcystin toxin is bound to Cys-273 through a thioether bond.
      Molecular Weight~37 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PP11 - 2051935 2051935
      Anti-PP11 - 2273994 2273994
      Anti-PP11 - JBC1785478 JBC1785478
      Anti-PP11 - NG1602926 NG1602926
      Anti-PP11 - NG1660337 NG1660337
      Anti-PP11 - NG1876737 NG1876737
      Anti-PP11 - NG1948814 NG1948814
      Anti-PP1γ1 -2567065 2567065
      Anti-PP1γ1 -2594355 2594355

      References

      Reference overviewPub Med ID
      High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1.
      Zhou, X; Wang, H; Burg, MB; Ferraris, JD
      American journal of physiology. Renal physiology 305 F362-9 2013

      Show Abstract
      23720348 23720348

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies