A part of MilliporeSigma

MABE172 | Anti-PML Isoform V Antibody, clone 18B5.2

100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABE172
      DescriptionAnti-PML Isoform V Antibody, clone 18B5.2
      Alternate Names
      • PML-5
      • PML-V
      • Protein PML isoform V
      • Promyelocytic leukemia protein isoform V
      • RING finger protein 71 isoform V
      • TRIM19beta
      • Tripartite motif-containing protein 19 isoform V
      Background InformationProtein PML (UniProt P29590; also known as Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19) is encoded by the PML (also known as MYL, PP8675, RNF71, TRIM19) gene (Gene ID 5371) in human. The human PML gene/protein adopted its name when it was originally discovered as an RARα fusion protein caused by chromosomal translocation in acute promyelocytic leukemia (APL) patients. In normal cells, the PML protein interacts with multiple protein partners in the nucleus to form PML nuclear bodies (PML-NBs) that mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and antiviral defense. At least 12 PML isoforms are generated by alternative splicings, including six nuclear isoforms (PML-I to PML-VI) and the cytoplasmic isoform PML-VII (a.k.a. PML-VIIB), other spliced isoforms wihtout exon 6-coding sequence (a.a. 467-552) are likewise expected to be cytoplasmic due to the abscence of nuclear localization signal (NLS) encoded by exon 6. All isoforms share the same N-terminal region (a.a. 1-419) containing the RBCC/tripartite motif and may have related functions due to their common functional RBCC/TRIM domain, while increasing evidences also suggest that each PML isoform possesses distinct functions mediated by its specific C-terminal sequence. Isoform PML-1 (a.k.a. PML-I, TRIM19alpha; UniProt P29590-1) and PML-5 (a.k.a. PML-V or TRIM19beta; UniProt P29590-2) share the same N-terminal 570-a.a. sequence (encoded by exons 1 to 7a), however PML-1 derives its remaining C-terminal sequence (a.a. 571-882) from exons 8a & 9, while PML-V derives its (a.a. 571-611) from exon 7b with a different reading frame as that of PML-II.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-PML Isoform V Antibody, clone 18B5.2 is an antibody against PML Isoform V for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 10 µg/mL from a representative lot detected the expression of exogenously transfected human PML isoform V (PML-5), but not isoforms PML-1, -2, -3, -4, or -6, in lysates from transfected HEK293 cells (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).
      Immunocytochemistry Analysis: 40 µg/mL from a representative lot immunostained 10% neutral-buffered formalin-fixed HEK293 cells transfected with human PML isoform V by non-fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).
      Immunocytochemistry Analysis: 10 µg/mL from a representative lot immunostained 4% paraformaldehyde-fixed HEK293 cells transfected with human PML isoform V by fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).
      Biological Information
      ImmunogenGST-tagged recombinant human PML-5 C-terminal fragment.
      EpitopeC-terminal region.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 18B5.2 reacts with PML spliced isoform V (PML-5), but not isoforms PML-1, -2, -3, -4, or -6.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PML
      • MYL
      • PP8675
      • RNF71
      • TRIM19
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~75 kDa observed. 67.47 kDa calculated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from PML-5 transfected HEK293 cells.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected the exogenously expressed human PML-5 in 10 µg of lysate from transfected HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information