A part of MilliporeSigma

05-366 | Anti-PLCγ-1 Antibody, clone B-6-4

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, R, Rb IP, WB M Purified Monoclonal Antibody
      Catalogue Number05-366
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PLCγ-1 Antibody, clone B-6-4
      Product Information
      PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPurified soluble phospholipase C (PLC) g-1 from bovine brain
      Concentration1 mg/mL
      SpecificityPLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PLCG1
      • PLC1
      • PLCgamma1
      • PLC148
      • PLC-II
      • PLC-148
      • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
      • PLC-gamma-1
      • inositoltrisphosphohydrolase
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
      COFACTOR: Calcium.
      SIZE: 1290 amino acids; 148532 Da
      SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).
      PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).
      SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.
      Molecular Weight~135 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PLC #947;-1, clone B-6-4 - 23344 23344
      Anti-PLC g-1, clone B-6-4 - DAM1564508 DAM1564508
      Anti-PLC γ-1, clone B-6-4 -2570805 2570805
      Anti-PLC γ-1, clone B-6-4 -2608717 2608717
      Anti-PLC γ-1, clone B-6-4 -2664936 2664936
      Anti-PLCgamma;-1, clone B-6-4 - 14269 14269
      Anti-PLCgamma;-1, clone B-6-4 - 17307 17307
      Anti-PLCgamma;-1, clone B-6-4 - 18606 18606

      References | 11 Available | See All References

      Reference overviewApplicationPub Med ID
      Aging and a peripheral immune challenge interact to reduce mature brain-derived neurotrophic factor and activation of TrkB, PLCgamma1, and ERK in hippocampal synaptoneurosomes.
      Cortese, GP; Barrientos, RM; Maier, SF; Patterson, SL
      The Journal of neuroscience : the official journal of the Society for Neuroscience 31 4274-9 2011

      Show Abstract
      Western Blotting21411668 21411668
      The ubiquitously expressed Csk adaptor protein Cbp is dispensable for embryogenesis and T-cell development and function.
      Dobenecker, MW; Schmedt, C; Okada, M; Tarakhovsky, A
      Molecular and cellular biology 25 10533-42 2005

      Show Abstract Full Text Article
      Western Blotting16287865 16287865
      Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.
      Roli Prasad, Jayashree Venkatasubramanian, Milen Amde, Mrinalini Chatta Rao
      Digestive diseases and sciences 49 1318-26 2004

      Show Abstract
      15387363 15387363
      Protein kinase C regulates integrin-induced activation of the extracellular regulated kinase pathway upstream of Shc
      Miranti, C. K., et al
      J Biol Chem, 274:10571-81 (1999) 1999

      Immunoprecipitation, Immunoblotting (Western)10187852 10187852
      SH2-B is required for nerve growth factor-induced neuronal differentiation.
      Rui, L, et al.
      J. Biol. Chem., 274: 10590-4 (1999) 1999

      Show Abstract
      Immunoblotting (Western)10187854 10187854
      Tyrosine phosphorylation and recruitment of ZAP-70 to the CD3-TCR complex are defective after CD2 stimulation
      Hubert, P., et al
      J Immunol, 157:4322-32 (1996) 1996

      Immunoprecipitation8906806 8906806
      T cell activation-dependent association between the p85 subunit of the phosphatidylinositol 3-kinase and Grb2/phospholipase C-gamma 1-binding phosphotyrosyl protein pp36/38.
      Fukazawa, T, et al.
      J. Biol. Chem., 270: 20177-82 (1995) 1995

      Show Abstract
      Immunoprecipitation, Immunoblotting (Western)7544353 7544353
      Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP.
      Park, D and Rhee, S G
      Mol. Cell. Biol., 12: 5816-23 (1992) 1992

      Show Abstract
      1333046 1333046
      CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.
      Park, D J, et al.
      Proc. Natl. Acad. Sci. U.S.A., 88: 5453-6 (1991) 1991

      Show Abstract
      1828897 1828897
      Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
      Meisenhelder, J, et al.
      Cell, 57: 1109-22 (1989) 1989

      Show Abstract
      2472219 2472219

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      Life Science Research > Antibodies and Assays > Primary Antibodies