ST1042 Anti-PKD2 Rabbit pAb

ST1042
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1042-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~105 kDa PKD2 protein in HEK293 cells.
          Catalogue NumberST1042
          Brand Family Calbiochem®
          SynonymsAnti-Protein Kinase D2
          Application Data
          Panel A: Detection of human PKD2 by immunoblotting. Samples: Whole cell lysate (30 µg) from untransfected HEK 293 cells (Ctrl, left lane), HEK 293 cells transfected with PKD3-GFP fusion protein (~136 kDa, middle lane), or HEK 293 cells transfected with PKD1-GFP fusion protein (~136 kDa, right lane). Primary antibody: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:1000) Detection: chemiluminescence. Panel B: Detection of human PKD2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate (200 µg protein) from HEK 293 cells. Antibody for IP: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:100). Immunoblotting conditions: same as above.
          References
          ReferencesRey, O., et al. 2003. Biochem. Biophys. Res. Commun. 302, 817.
          Sturany, S., et al. 2002. J. Biol. Chem. 277, 29431.
          Sturany, S., et al. 2001. J. Biol. Chem. 276, 3310.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Positive controlHEK 293 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:500-1:5000)
          Immunoprecipitation (1-4 µg/mg lysate)
          Application CommentsThis antibody does not cross-react with PKD1 or PKD3. Antibody should be titrated for optimal use in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human PKD2
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PKD2 Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PKD2 Rabbit pAb Certificates of Analysis

          TitleLot Number
          ST1042

          References

          Reference overview
          Rey, O., et al. 2003. Biochem. Biophys. Res. Commun. 302, 817.
          Sturany, S., et al. 2002. J. Biol. Chem. 277, 29431.
          Sturany, S., et al. 2001. J. Biol. Chem. 276, 3310.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-March-2009 JSW
          SynonymsAnti-Protein Kinase D2
          ApplicationImmunoblotting (1:500-1:5000)
          Immunoprecipitation (1-4 µg/mg lysate)
          Application Data
          Panel A: Detection of human PKD2 by immunoblotting. Samples: Whole cell lysate (30 µg) from untransfected HEK 293 cells (Ctrl, left lane), HEK 293 cells transfected with PKD3-GFP fusion protein (~136 kDa, middle lane), or HEK 293 cells transfected with PKD1-GFP fusion protein (~136 kDa, right lane). Primary antibody: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:1000) Detection: chemiluminescence. Panel B: Detection of human PKD2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate (200 µg protein) from HEK 293 cells. Antibody for IP: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:100). Immunoblotting conditions: same as above.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~105 kDa PKD2 protein.
          BackgroundProtein kinase D2 (PKD2) is a serine/threonine protein kinase and a member of PKD family, which also includes PKD/PKD1/PKCµ and PKD3/PKCν. PKDs are a newly identified protein kinase family with structural, enzymological and regulatory properties different from protein kinase C (PKC) family. PKDs participate in the signal transduction pathway and are potential regulators of cell proliferation and apoptosis. They are stimulated by diacyl-glycerol (DAG), and are substrates for classical PKCs like PKCα, PKCε and PKCη. Therefore, PKD stimulation by DAG is partially mediated through PKCs. PKD2 mRNA is widely expressed in human and murine tissue and codes for a ~105 kDa protein. It is stimulated by both phorbol ester and growth factors and is autophosphorylated upon stimulation. PKD2 is predominantly located in the cytoplasm and, as opposed to other members of the PKD family, does not accumulate in the nucleus upon induction. This suggests that PKD2 mediates physiological responses different from other members of the PKD family.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human PKD2
          IsotypeIgG
          Specieshuman
          Positive controlHEK 293 cells
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody does not cross-react with PKD1 or PKD3. Antibody should be titrated for optimal use in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw.
          Toxicity Highly Toxic
          ReferencesRey, O., et al. 2003. Biochem. Biophys. Res. Commun. 302, 817.
          Sturany, S., et al. 2002. J. Biol. Chem. 277, 29431.
          Sturany, S., et al. 2001. J. Biol. Chem. 276, 3310.