05-154 Anti-PKCα Antibody, clone M4

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, R, Rb IP, NEUT, RIA, WB M Purified Monoclonal Antibody
      Catalogue Number05-154
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PKCα Antibody, clone M4
      Alternate Names
      • aging-associated gene 6
      • protein kinase C alpha type
      • protein kinase C, alpha
      Background InformationProtein Kinase c alpha (PKC alpha) is a 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell-cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy-terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
      Product Information
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in storage buffer containing (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide).
      ApplicationAnti-PKCα Antibody, clone M4 is an antibody against PKCα for use in IP, NEUT, RIA & WB.
      Key Applications
      • Immunoprecipitation
      • Neutralizing
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated PKCα from an A431 RIPA cell lysate and a bovine brain extract.

      An independent laboratory showed that 20 μg of this antibody can inhibit >50% of the kinase activity of 20 ng PKCα (Leach, K.L., 1998).
      Biological Information
      ImmunogenPKCα purified from rabbit brain cytosol.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificitySpecific for PKCα. MW: ~82 kDa.
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
      Gene Symbol
      • PRKCA
      • PKC-A
      • AAG6
      • PKC-alpha
      • MGC129901
      • MGC129900
      • PRKACA
      • PKCA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 672 amino acids; 76764 Da
      SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
      SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight~82 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated in A431 RIPA cell lysate or bovine brain cytosolic extract.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected PKCα from a human A431 RIPA cell lysate. Previous lots detected PKCα in bovine brain cytosolic extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKC#945;, clone M4 - DAM1828779DAM1828779
      Anti-PKC, clone M4 - 22103982210398
      Anti-PKC, clone M4 - DAM1695851DAM1695851
      Anti-PKC, clone M4 - DAM1734743DAM1734743
      Anti-PKC, clone M4 - DAM1770256DAM1770256
      Anti-PKC, clone M4 - DAM1791288DAM1791288
      Anti-PKC, clone M4 - JBC1873434JBC1873434
      Anti-PKCa, clone M4 - DAM1530428DAM1530428
      Anti-PKCalpha, clone M4 - DAM1632056DAM1632056
      Anti-PKCalpha;, clone M4 - 1400814008


      Reference overviewApplicationSpeciesPub Med ID
      Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice.
      Fujita, H; Aoki, H; Ajioka, I; Yamazaki, M; Abe, M; Oh-Nishi, A; Sakimura, K; Sugihara, I
      PloS one  9  e86679  2014

      Show Abstract
      24475166 24475166
      Transgenic overexpression of PKCε in the mouse prostate induces preneoplastic lesions.
      Benavides, F; Blando, J; Perez, CJ; Garg, R; Conti, CJ; DiGiovanni, J; Kazanietz, MG
      Cell cycle (Georgetown, Tex.)  10  268-77  2011

      Show Abstract Full Text Article
      Western BlottingMouse21224724 21224724
      The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons.
      de Melo, J; Peng, GH; Chen, S; Blackshaw, S
      Development (Cambridge, England)  138  2325-36  2011

      Show Abstract Full Text Article
      21558380 21558380
      Differential distribution of exchange proteins directly activated by cyclic AMP within the adult rat retina.
      C M Whitaker,N G F Cooper
      Neuroscience  165  2010

      Show Abstract Full Text Article
      19883736 19883736
      A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers.
      Hennessy, BT; Lu, Y; Gonzalez-Angulo, AM; Carey, MS; Myhre, S; Ju, Z; Davies, MA; Liu, W; Coombes, K; Meric-Bernstam, F; Bedrosian, I; McGahren, M; Agarwal, R; Zhang, F; Overgaard, J; Alsner, J; Neve, RM; Kuo, WL; Gray, JW; Borresen-Dale, AL; Mills, GB
      Clinical proteomics  6  129-51  2010

      Show Abstract Full Text Article
      21691416 21691416
      Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.
      Levin, VA; Panchabhai, SC; Shen, L; Kornblau, SM; Qiu, Y; Baggerly, KA
      Journal of proteome research  9  179-91  2010

      Show Abstract
      Western BlottingHuman19894763 19894763
      The long noncoding RNA RNCR2 directs mouse retinal cell specification.
      Rapicavoli, NA; Poth, EM; Blackshaw, S
      BMC developmental biology  10  49  2010

      Show Abstract
      Immunofluorescence20459797 20459797
      Developmental sources of conservation and variation in the evolution of the primate eye.
      Michael A Dyer,Rodrigo Martins,Manoel da Silva Filho,José Augusto P C Muniz,Luiz Carlos L Silveira,Constance L Cepko,Barbara L Finlay
      Proceedings of the National Academy of Sciences of the United States of America  106  2009

      Show Abstract Full Text Article
      19451636 19451636
      Role for protein kinase C-alpha in keratinocyte growth arrest.
      Jerome-Morais, A; Rahn, HR; Tibudan, SS; Denning, MF
      The Journal of investigative dermatology  129  2365-75  2009

      Show Abstract
      19340015 19340015
      Phorbol ester-stimulated NF-kappaB-dependent transcription: roles for isoforms of novel protein kinase C.
      Neil S Holden, Paul E Squires, Manminder Kaur, Rosemary Bland, Carol E Jones, Robert Newton
      Cellular signalling  20  1338-48  2008

      Show Abstract
      18436431 18436431

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      Life Science Research > Antibodies and Assays > Primary Antibodies