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MABN1052 | Anti-PITX3 Antibody, clone EPR8868, rabbit monoclonal

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC Rb Unpurified Monoclonal Antibody
      Catalogue NumberMABN1052
      DescriptionAnti-PITX3 Antibody, clone EPR8868, rabbit monoclonal
      Alternate Names
      • Pituitary homeobox 3
      • Homeobox protein PITX3
      • Paired-like homeodomain transcription factor 3
      Background InformationPituitary homeobox 3, also named Homeobox protein PITX3 or Paired-like homeodomain transcription factor 3 with genes names PITX3 or PTX3 is a homebox transcription factor that is essential for the differentiation and maintenance of the meso-diencephalic dopaminergic (mdDA) neurons during development and for their long term survival and maintenance during adulthood. Several variants of this gene have been associated with human Parkinson's disease (PD), and lack of PITX3 in mice causes the preferential loss of substantia nigra pars compacta (SNc) mdDA neurons that are most affected in PD. Thus the study of PITX3 expression and the genes it helps mediate is of critical importance in understanding the pathology of Parkinson’s disease. PTX3 activates NR4A2/NURR1 mediated transcription of many genes essential for mdDA growth and survival including SLC6A3, TH, and DRD2. Interestingly PITX3 also plays important roles in the eye where it is essential for proper lens development and fiber cell differentiation and the induction of specific crystalline proteins. Defects in the gene are the cause of numerous cataract or developmental eye related abnormalities. Highly expressed in the developing eye and dopaminergic regions in the brain, PITX3 is was recently shown to be a GDNF responsive gene that is essential for activating the expression of brain-derived neurotrophic factor (BDNF) in mdDA neuron subpopulations during embryogenesis. The loss of BDNF expression is correlated with the increased apoptotic cell death of mdDA neuronal subpopulations in mice lacking PITX3. The regulation of GDNF, PITX3, and BDNF expression also persists in the adult brain, and this suggests that any impairment or disruption of the regulatory interaction between these three factors may contribute to the loss of mdDA neurons and subsequent disease pathology.
      Product Information
      • 293T, Y79, and U87-MG cell lysates
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A 1:10-100 dilution from a representative lot detected PITX3 in 293T cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human PITX3.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PITX3
      • PTX3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~32 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 293T, Y79, and U87-MG cell lysates.

      Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected PITX3 in 293T, Y79, and U87-MG cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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