A part of MilliporeSigma

AB2955 | Anti-PIST Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Rhesus Macaque, Ca, Ox WB, IH(P) Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB2955
      DescriptionAnti-PIST Antibody
      Alternate Names
      • Golgi-associated PDZ and coiled-coil motif-containing protein
      • CFTR-associated ligand
      • PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
      • PDZ protein interacting specifically with TC10
      Background InformationPDZ domain protein interacting specifically with TC10 (PIST) is also known as Golgi-associated PDZ and coiled-coil motif-containing protein, a protein consisting of 462 amino acids. PIST has an important role in the trafficking of various intracellular proteins and degradation within the cytoplasm in association with the Golgi apparatus. It is necessary in frizzled protein transport and transporting the cystic fibrosis transmembrane conductance regulator (CTFR) to the plasma membrane. PIST has two N-terminal coiled-coil domains in addition to its C-terminal PDZ domain, and is ubiquitously expressed in tissue including synaptosomal and postsynaptic density fractions. It has been suggested that PIST may play a role in autophagy and also neurodegeneration. Overexpression of PIST is likely to be causal in CTFR intracellular retention leading to lysosomal degradation. The involvement of PIST within Chromosomal aberrations has been observed in glioblastoma multiform.
      Product Information
      FormatAffinity Purified
      • Mouse brain lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      ApplicationAnti-PIST Antibody detects level of PIST & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot (SNAP ID) Analysis: 1 µg/mL of this antibody has been shown to detect PIST in 10 µg of mouse brain lysate.

      Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect PIST in normal human brain tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse PIST near the C-terminus.
      EpitopeNear the C-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThe antibody recognizes PIST near the C-terminus.
      Species Reactivity
      • Mouse
      • Human
      • Rhesus Macaque
      • Canine
      • Ox
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rhesus macaque, canine, and ox based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PIST
      • FIG
      • CAL
      • GOPC1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.

      SUBUNIT STRUCTURE: Homooligomer. Interacts with STX6 and GOLGA3 by similarity. Interacts with ACCN3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2.

      SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › dendrite. Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.

      TISSUE SPECIFICITY: Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia.

      DEVELOPMENTAL STAGE: In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level).

      DOMAIN: The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 (by similarity).

      The PDZ domain mediates interaction with ADRB1 by similarity. Mediates also interactions with FZD5, FZD8, ACCN3, GRID2, CFTR, CLCN3.

      DISRUPTION PHENOTYPE: Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes.

      SEQUENCE SIMILARITIES: Contains 1 PDZ (DHR) domain.
      Molecular Weight ~ 55kDa Observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected PIST in 10 µg of mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information