A part of MilliporeSigma

MABE1003 | Anti-PIF1 Antibody, clone 12A11.1

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB M Purified Monoclonal Antibody
      Catalogue NumberMABE1003
      DescriptionAnti-PIF1 Antibody, clone 12A11.1
      Alternate Names
      • ATP-dependent DNA helicase PIF1
      • PIF1
      • DNA repair and recombination helicase PIF1
      • PIF1/RRM3 DNA helicase-like protein
      Background InformationATP-dependent DNA helicase PIF1 (EC; UniProt Q9H611; also known as DNA repair and recombination helicase PIF1, Petite integration frequency 1, PIF1 5'-to-3' DNA helicase homolog, PIF1/RRM3 DNA helicase-like protein) is encoded by the PIF1 (also known as PIF, C15orf20) gene (Gene ID 80119) in human. Human PIF1 helicase and the yeast ortholog share common substrates in vitro, exhibiting specificity toward telomeric DNA, synthetic stalled DNA replication fork-like structures, especially G4-DNA. PIF1 functions to maintain undisrupted DNA replication fork progression during normal cycling conditions and to support resumption of DNA synthesis after prolonged S-phase arrest. Cancer cells are reported to exhibit increased dependency on PIF for DNA replication. PIF1-depletion results in a combination of apoptosis, reduced survival, and hypersensitivity to therapeutic DNA replication inhibitors in several cancer cell lines independent of p53 status.
      Product Information
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-PIF1 Antibody, clone 12A11.1 is an antibody against PIF1 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human PIF1.
      EpitopeC-terminal half
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with isoforms 1 (isoform alpha) and isoform 4, but not isoform 2. Reactivity toward isoform 3 (isoform beta) has not been determined.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PIF1
      • C15orf20
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed. 69.8 kDa (isoform 1), 77.1 kDa (isoform 3), 63.9 kDa (isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PIF1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information