A part of MilliporeSigma

MAB2148-C | Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free)

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, ICC, IP, IHC, ELISA, IF M Purified Monoclonal Antibody
      Catalogue NumberMAB2148-C
      Replaces04-1074, MAB2148
      DescriptionAnti-PECAM-1 Antibody, clone P2B1 (Ascites Free)
      Alternate Names
      • Platelet endothelial cell adhesion molecule
      • PECAM-1
      • EndoCAM
      • GPIIA
      • PECA1
      • CD31
      Background InformationPECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry
      • ELISA
      • Immunofluorescence
      Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et al. (2007). Exp Biol Med. 232(6):833-843.).

      Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).

      ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
      Biological Information
      ImmunogenWhole cells expressing human PECAM-1.
      Concentration1 mg/mL
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PECAM1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in human PBMCs.

      Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies