A part of MilliporeSigma

ST1643 | Anti-PDK2 Mouse mAb (2G1)

ST1643
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      ST1643-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          100 μg
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          Description
          OverviewRecognizes the ~40 kDa PDK2 protein in U-2 OS cells.
          Catalogue NumberST1643
          Brand Family Calbiochem®
          SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
          Application Data
          Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlU-2 OS cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.8 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
          Clone2G1
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-November-2010 RFH
          SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.8 µg/ml)
          Application Data
          Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
          BackgroundThe PDK2 protein inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 α subunit, thus contributing to the regulation of glucose metabolism.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
          Clone2G1
          IsotypeIgG2a
          Specieshuman
          Positive controlU-2 OS cells
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review