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CBL407F | Anti-PCNA Antibody, clone PC10, FITC conjugated

CBL407F
100 assays  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Inv, Vrt FC M FITC Monoclonal Antibody
      Description
      Catalogue NumberCBL407F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PCNA Antibody, clone PC10, FITC conjugated
      Alternate Names
      • Proliferating Cell Nuclear Antigen
      • DNA Polymerase delta Processivity Factor
      Background InformationProliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.
      References
      Product Information
      FormatFITC
      Control
      • POSITIVE CONTROL: Tonsil or reactive lymph node.
      PresentationThe conjugate is supplied as a 100 test/1 mL vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      ApplicationUse Anti-PCNA Antibody, clone PC10, FITC conjugated (mouse monoclonal antibody) validated in FC to detect PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor.
      Key Applications
      • Flow Cytometry
      Application NotesImmunostaining of malignant tumours with low and high proliferation on paraffin wax embedded sections

      Study of cell proliferation with PHA stimulated peripheral blood lymphocytes and various neoplasms

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ClonePC10
      HostMouse
      SpecificityThis monoclonal reacts with a nuclear antigen that is expressed by proliferating cells in all phases of the active cell cycle from all vertebrate species and insects.
      IsotypeIgG2a
      Species Reactivity
      • Invertebrates
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
      SIZE: 261 amino acids; 28769 Da
      SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
      DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
      SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED MONOCLONAL ANTIBODY - 2450123 2450123
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN -2630255 2630255
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN -2650808 2650808
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN -2777860 2777860
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED MONOCLONAL ANTIBODY - 2197216 2197216
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED MONOCLONAL ANTIBODY - 2208774 2208774
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED MONOCLONAL ANTIBODY - 2224391 2224391
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED MONOCLONAL ANTIBODY - 2282156 2282156

      References

      Reference overviewPub Med ID
      PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines.
      Coltrera, M D and Gown, A M
      J. Histochem. Cytochem., 39: 23-30 (1991) 1991

      Show Abstract
      1670579 1670579
      Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
      Waseem, N H and Lane, D P
      J. Cell. Sci., 96 ( Pt 1): 121-9 (1990) 1990

      Show Abstract
      1695635 1695635
      Proliferating cell nuclear antigen (PCNA)/cyclin in activated human T lymphocytes.
      Kurki, P, et al.
      J. Immunol., 138: 4114-20 (1987) 1987

      Show Abstract
      2884260 2884260
      Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies.
      Robbins, B A, et al.
      Arch. Pathol. Lab. Med., 111: 841-5 (1987) 1987

      Show Abstract
      2888448 2888448
      Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells.
      Celis, J E, et al.
      Leuk. Res., 8: 143-57 (1984) 1984

      6143860 6143860

      Data Sheet

      Title
      MOUSE ANTI-HUMAN PROLIFERATION CELL NUCLEAR ANTIGEN FITC CONJUGATED

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      Alternative Format

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies