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MAB4078 | Anti-PCNA Antibody, clone 131-11912

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, M, R, Rb, F, Vrt IF, ICC, IH(P) M Ascites Monoclonal Antibody
      Catalogue NumberMAB4078
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PCNA Antibody, clone 131-11912
      Alternate Names
      • Proliferating Cell Nuclear Antigen
      • DNA Polymerase delta Processivity Factor
      Product Information
      PresentationAscites fluid containing no preservatives
      ApplicationAnti-PCNA Antibody, clone 131-11912 is a mouse monoclonal antibody for detection of PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor & has been validated in IF, ICC,IHC(P).
      Key Applications
      • Immunofluorescence
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry on formalin fixed, deparaffinized tissue sections
      Frozen tissue sections.
      * Do not use Serum in blocking steps.

      Also see http://ajp.amjpathol.org/cgi/content/abstract/141/6/1453 for important reactivity information on 19A2 in paraffin fixed tissues.

      Final working dilutions must be determined by end user.


      The following histochemical immunoperoxidase protocol has been optimized for use with MAB4078.

      METHODOLOGY: This method is used with standard streptavidin or ABC immunoperoxidase methods using a DAB substrate run at room temperature with paraffin or frozen sections, smears or cytospins.

      NOTE: Optimal fixation time for paraffin processed tissue fixed in 10% Neutral Buffered Formalin is 12-14 hours.


      1. 3% Hydrogen Peroxide: commercially available.

      2. PBS

      3. Ethyl Alcohol: histology grade or equivalent

      4. Methanol: histology grade or equivalent

      5. Acetone: histology grade or equivalent

      6. Xylene (histology grade) or Xylene Substitute (Hemo-D)

      7. Mounting Media: Krystalon (Harleco) or equivalent

      8. Gill #1 Hematoxylin: Sigma #GHS-1-32 or equivalent

      9. Goat Anti-Mouse Biotin

      10. Streptavidin HRP (optional reagent)

      11. 3,3'Diaminobenzidine tetrahydrochloride (DAB): Sigma #D-5637 or equivalent

      12. ABC: Vector Kit #PK4000 or equivalent


      1. Primary antibody: Prepare a stock solution of antibody in PBS

      2. Goat Anti-Mouse Biotin: Approximate dilution of 1:100 in PBS (10 mg/test)

      3. Streptavidin: 5 mL stock solution and 995 mL PBS (1/200 dilution). Let sit 15 minutes before use (yield as a volume sufficient for ~20 slides) OR if using ABC kit from Vector follow as instructed.

      4. DAB: Reconstitute 1.5 mg of powder with 100 mL of PBS. Activate 15 minutes prior to use by adding 100 mL 3% H2O2 (yields a volume sufficient for ~20 slides). WARNING: DAB is a possible carcinogen. Avoid contact with skin.

      5. PBS: Using a graduated cylinder, reconstitute with 500 mL of distilled water.

      6. Alcohol Baths: All dilutions are alcohols made with distilled water.



      (cut at 4-5 microns, 10% Neutral Buffered Formalin fixed):

      1. Place slides in 62°C oven for 15 minutes.

      2. Place slides in Hemo-D, or xylene, for 15 minutes.

      3. De-paraffinize slides through baths of: A) xylene/100% ethanol 1:1 (or Hemo-D/100% ethanol 1:1), B) 100% ethyl alcohol x2, C) 95% ethyl alcohol, D) 90% ethyl alcohol, E) 80% ethyl alcohol, F) 70% ethyl alcohol x2 (2 minutes each bath).

      4. Place slides in running cool tap water for 5 minutes.

      5. Shake off excess water. Place slides on incubation racks held at room temperature. Circle sections with PAP pen (Newcomer, Inc.) or diamond pen to isolate section.

      6. Flood sections with primary antibody and incubate for 35 - 40 minutes.

      7. Wipe off excess PBS. Flood sections with Goat Anti-Mouse Biotin reagent and incubate for 35-40 minutes.

      8. Wash with PBS 3-5 min

      9. Wash again with PBS 3-5 min.

      10. Wipe off excess PBS. Flood sections with streptavidin-HRP and incubate for 10 minutes OR flood sections with ABC and incubate for 35 - 40 minutes.

      11. Wash with PBS 3-5 min.

      12. Wipe off excess PBS. Flood sections with DAB and incubate for 6-8 minutes.

      13. Place slides in cool running tap water for 5 minutes.

      14. Place slides in Gills #1 hematoxylin for 90 seconds.

      15. Place slides in cool running tap water for 5 minutes.

      16. Dehydrate slides through baths of A) 70% ethyl alcohol, B) 80% ethyl alcohol, C) 90% ethyl alcohol, D) 95% ethyl alcohol x2, E) 100% ethyl alcohol x2, F) xylene/100% ethanol 1:1 (or Hemo-D/100% ethanol 1:1) x2, G) xylene or Hemo-D x2 (2 minutes each bath.

      17. Mount slides using mounting media.

      FROZEN SECTIONS (cut at 8 microns): Pull sections from freezer (or cut fresh), air dry at room temperature (or under a fan for 15 minutes), fixed in 4ºC acetone for 4 minutes, air dry for 30 minutes at room temperature (or under a fan for 15 minutes), then follow above procedure beginning at #5.

      SMEARS: Methanol fix for 30 minutes at 4ºC, then follow above procedure beginning at #5.

      CYTOSPINS: Cell lines and other cell populations prepared by resuspending, in each well, ~40,000 cells using 500 mL 1% Normal Goat Serum per well. Centrifuge for 6 minutes at 1200 RPM. Air dry for 30 minutes at room temperature (or under a fan for 15 minutes). If tumor cells are being screened, fix in RT acetone for 2 - 3 minutes. If peripheral blood preps are being screened, fix in 100% methanol for 20 minutes. Air dry the slides for 30 minutes at room temperature (or 15 minutes under a fan) and begin at #5 above.

      NOTE: When staining using the PCNA antibodies as the primary DO NOT use Normal Goat Serum or any other serum blocking step.
      Biological Information
      ImmunogenPurified PCNA
      SpecificitySpecific for PCNA a 36 kDa proliferation associated antigen.
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Fish
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
      SIZE: 261 amino acids; 28769 Da
      SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
      PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
      DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
      SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-PCNA 2479772
      MOUSE ANTI-PCNA (Proliferating Cell Nuclear Antigen) MONOCLONAL ANTIBODY -
      MOUSE ANTI-PCNA (Proliferating Cell Nuclear Antigen) MONOCLONAL ANTIBODY - 2194020 2194020
      MOUSE ANTI-PCNA (Proliferating Cell Nuclear Antigen) MONOCLONAL ANTIBODY - 2275544 2275544
      MOUSE ANTI-PCNA -2594306 2594306
      MOUSE ANTI-PCNA -2676267 2676267


      Reference overviewPub Med ID
      Temozolomide PLGA microparticles: a new protocol for treatment of glioma in rats.
      Zhang YH, Zhang H, Liu JM, Yue ZJ
      Medical oncology (Northwood, London, England) 28 901-6. Epub 2010 Apr 16. 2011

      Show Abstract
      20396980 20396980
      Wood JP, Chidlow G, Tran T, Crowston J, Casson RJ
      Invest Ophthalmol Vis Sci 2010

      Show Abstract
      20181845 20181845
      Temozolomide/PLGA microparticles plus vatalanib inhibits tumor growth and angiogenesis in an orthotopic glioma model.
      Yu-Hui Zhang,Zhi-Jian Yue,He Zhang,Gu-Sheng Tang,Yang Wang,Jian-Min Liu
      European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V 76 2010

      Show Abstract
      20816959 20816959
      Drosophila CAF-1 regulates HP1-mediated epigenetic silencing and pericentric heterochromatin stability.
      Huang H, Yu Z, Zhang S, Liang X, Chen J, Li C, Ma J, Jiao R
      J Cell Sci 123 2853-61. Epub 2010 Jul 27. 2010

      Show Abstract
      20663913 20663913
      Decreased neurogenesis in aged rats results from loss of granule cell precursors without lengthening of the cell cycle.
      Ana Olariu,Kathryn M Cleaver,Heather A Cameron
      The Journal of comparative neurology 501 2007

      Show Abstract
      17278139 17278139
      Determination of key aspects of precursor cell proliferation, cell cycle length and kinetics in the adult mouse subgranular zone.
      Mandyam, CD; Harburg, GC; Eisch, AJ
      Neuroscience 146 108-22 2007

      Show Abstract Full Text Article
      17307295 17307295
      Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer.
      Siitonen, S M, et al.
      Am. J. Pathol., 142: 1081-9 (1993) 1993

      Show Abstract
      7682759 7682759

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