NA03 Anti-PCNA (Ab-1) Mouse mAb (PC10)

NA03
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R, Yeast, Zebrafish M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NA03-200UG
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          Plastic ampoule 200 μg
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          Description
          OverviewRecognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
          Catalogue NumberNA03
          Brand Family Calbiochem®
          SynonymsAnti-Proliferating Cell Nuclear Antigen
          References
          ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
          Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Paraffin Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Not Immunoprecipitation
          Application NotesImmunoblotting (2.5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (not recommended with unconjugated antibody, see comments)
          Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
          Application CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant PCNA
          EpitopeWithin amino acids 112-121
          ClonePC10
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          • Yeast
          • Zebrafish
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PCNA (Ab-1) Mouse mAb (PC10) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PCNA (Ab-1) Mouse mAb (PC10) Certificates of Analysis

          TitleLot Number
          NA03

          References

          Reference overview
          Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
          Miyachi, K., et al. 1987. J. Immunol. 121, 2228.

          Citations

          Title
          Goessling, et al. 2008. Developmental Biology 320, 161.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-September-2007 RFH
          SynonymsAnti-Proliferating Cell Nuclear Antigen
          ApplicationImmunoblotting (2.5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (not recommended with unconjugated antibody, see comments)
          Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
          BackgroundThe proliferating cell nuclear antigen (PCNA) is a 37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have auto antibodies against PCNA.
          HostMouse
          Immunogenrecombinant PCNA
          EpitopeWithin amino acids 112-121
          ClonePC10
          IsotypeIgG2a
          Specieshuman, mouse, rat, yeast, zebrafish
          Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
          Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
          Bravo, R., et al. 1987. Nature (London) 326, 515.
          Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
          Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
          CitationGoessling, et al. 2008. Developmental Biology 320, 161.