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DR1094 | Anti-PAX7 Mouse mAb (1E12)

DR1094
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      DR1094-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
          Catalogue NumberDR1094
          Brand Family Calbiochem®
          SynonymsAnti-Paired Box 7
          Application Data
          Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlNIH3T3 cells, Human stomach tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
          Clone1E12
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-November-2010 RFH
          SynonymsAnti-Paired Box 7
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (0.5 µg/ml)
          Application Data
          Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
          BackgroundThis protein is a member of the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These proteins play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
          Clone1E12
          IsotypeIgG2a
          Specieshuman, mouse
          Positive controlNIH3T3 cells, Human stomach tissue
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review