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MAB3290 | Anti-PARP Antibody, clone 7A10

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, M, R WB, FC M Purified Monoclonal Antibody
      Catalogue NumberMAB3290
      Replaces04-575; 04-576
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PARP Antibody, clone 7A10
      Alternate Names
      • Poly [ADP-ribose] polymerase 1
      • PARP-1
      • NAD(+) ADP-ribosyltransferase 1
      • ADPRT 1
      • Poly[ADP-ribose] synthase 1
      • ARTD1
      Background InformationPARP is a highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+ -dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in the apoptosis response of cells. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro.
      Product Information
      PresentationLiquid in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 ug/mL BSA, 50% glycerol and 0.03 sodium azide.
      ApplicationDetect PARP using this Anti-PARP Antibody, clone 7A10 validated for use in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blot (1:500-1:2000)
      Flow Cytometry (1:200-1:400)

      Optimal working dilutions must be determined by end user.

      For Western blotting, sonication and urea are necessary to break up PARP/DNA interactions. To do this, resuspend cells in 62.5 mM Tris-HCl, pH 6.8, 6 M urea, 10% glycerol, 2% SDS, 0.00125% bromophenol blue and 5% beta-mercaptoethanol, sonicate for 15 seconds on full power and incubate at 65°C for 15 minutes, and then follow standard Western blotting procedures. Untreated cells should show only full-length PARP, while treated cells will show both cleaved and full-length PARP.
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen.
      SpecificityThis antibody only recognizes full-length form (p116) of PARP-1.

      Epitope: Region before the cleavage site of PARP-1.
      Species Reactivity
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
      Gene Symbol
      • PARP1
      • poly(ADP-ribosyl)transferase
      • PARP-1
      • PARP
      • NAD
      • pADPRT-1
      • ADPRT1
      • Poly
      • ADPRT
      • PPOL
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
      SIZE: 1014 amino acids; 113084 Da
      SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
      PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
      SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers.
      MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number


      Reference overviewPub Med ID
      Poly-ADP ribosylation of PTEN by tankyrases promotes PTEN degradation and tumor growth.
      Li, N; Zhang, Y; Han, X; Liang, K; Wang, J; Feng, L; Wang, W; Songyang, Z; Lin, C; Yang, L; Yu, Y; Chen, J
      Genes & development 29 157-70 2015

      Show Abstract
      25547115 25547115
      SET protein accumulates in HNSCC and contributes to cell survival: Antioxidant defense, Akt phosphorylation and AVOs acidification.
      Andréia M Leopoldino,Cristiane H Squarize,Cristiana B Garcia,Luciana O Almeida,Cezar R Pestana,Lays M Sobral,Sérgio A Uyemura,Eloiza H Tajara,J Silvio Gutkind,Carlos Curti
      Oral oncology 48 2012

      Show Abstract
      22739068 22739068
      Apoptosis. Life and death decisions.
      Nicholson, Donald W and Thornberry, Nancy A
      Science, 299: 214-5 (2003) 2003

      12522239 12522239
      Different cleavage pattern for poly(ADP-ribose) polymerase during necrosis and apoptosis in HL-60 cells.
      Shah, G M, et al.
      Biochem. Biophys. Res. Commun., 229: 838-44 (1996) 1996

      Show Abstract
      8954981 8954981

      Data Sheet