04-575 | Anti-PARP-1 (p116/p25) Antibody, rabbit monoclonal

04-575
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB Rb Purified Monoclonal Antibody
      Description
      Catalogue Number04-575
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PARP-1 (p116/p25) Antibody, rabbit monoclonal
      Alternate Names
      • poly (ADP-ribose) polymerase-1
      • ARTD1
      Background InformationPoly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PARP-1 (p116/p25) Antibody detects level of PARP-1 (p116/p25) & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWB: 1:1000 IP: 1:80
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues before the cleavage site of human PARP-1. The antibody only recognizes full-length form (p116) of PARP-1.
      CloneY17
      HostRabbit
      SpecificityRecognizes Human PARP-1 (p116/p25).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman (positive control: Jurkat cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
      Gene Symbol
      • PARP1
      • NAD
      • Poly
      • PPOL
      • poly(ADP-ribosyl)transferase
      • PARP-1
      • PARP
      • pADPRT-1
      • ADPRT1
      • ADPRT
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
      SIZE: 1014 amino acids; 113084 Da
      SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
      SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers.
      MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PARP-1 (Rabbit monoclonal IgG) - 2421547 2421547
      Anti-PARP-1 (Rabbit monoclonal IgG) - 2216726 2216726
      Anti-PARP-1 (Rabbit monoclonal IgG) - 2228064 2228064
      Anti-PARP-1 (Rabbit monoclonal IgG) -2492237 2492237
      Anti-PARP-1 - 2024520 2024520
      Anti-PARP-1 - 2068406 2068406
      Anti-PARP-1 - DAM1567812 DAM1567812
      Anti-PARP-1 - DAM1724075 DAM1724075
      Anti-PARP-1 - NG1857565 NG1857565

      References

      Reference overviewPub Med ID
      An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells.
      Sousa, MM; Zub, KA; Aas, PA; Hanssen-Bauer, A; Demirovic, A; Sarno, A; Tian, E; Liabakk, NB; Slupphaug, G
      PloS one  8  e55493  2013

      Show Abstract
      23405159 23405159
      Identification of an intrinsic determinant critical for maspin subcellular localization and function.
      Dzinic, SH; Kaplun, A; Li, X; Bernardo, M; Meng, Y; Dean, I; Krass, D; Stemmer, P; Shin, N; Lonardo, F; Sheng, S
      PloS one  8  e74502  2013

      Show Abstract
      24278104 24278104
      Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
      Lazebnik, Y A, et al
      Nature, 371:346-7 (1994)  1994

      8090205 8090205
      Implications for the presence of transforming genes in gonadal tumors in two bivalve mollusk species
      Van Beneden, R J, et al
      Cancer Res, 53:2976-9 (1993)  1993

      8319204 8319204
      The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA.
      Ikejima, M, et al.
      J. Biol. Chem., 265: 21907-13 (1990)  1990

      Show Abstract
      2123876 2123876

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies