517310 Anti-P-Glycoprotein Mouse mAb (C219)

517310
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ht, H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      517310-500UL
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          Glass bottle 500 ul
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          Description
          OverviewRecognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
          Catalogue Number517310
          Brand Family Calbiochem®
          References
          ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
          Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
          Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
          Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
          Product Information
          DeclarationImmunoblotting Lee, G., et al. 2001. J. Pharmacol. Exp. Ther. 299, 204.
          FormLiquid
          FormulationIn PBS, 1% BSA.
          Positive controlCell lines or frozen tissue, such as liver or colon
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.
          Key Applications Immunocytochemistry
          Immunoprecipitation
          Paraffin Sections
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesFlow Cytometry (5-10 µg/1 x 10⁶ cells)
          Frozen Sections (5-10 µg/ml)
          Immunoblotting (1-10 µg/ml, see application references, see comments)
          Immunocytochemistry (see comments)
          Immunoprecipitation (see comments)
          Paraffin Sections (1-10 µg/ml)
          Application CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
          Biological Information
          ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
          ImmunogenHamster and Human
          CloneC219
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Hamster
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          517310

          References

          Reference overview
          Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
          Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
          Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
          Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.

          Citations

          Title
        • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
        • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-June-2013 JSW
          ApplicationFlow Cytometry (5-10 µg/1 x 10⁶ cells)
          Frozen Sections (5-10 µg/ml)
          Immunoblotting (1-10 µg/ml, see application references, see comments)
          Immunocytochemistry (see comments)
          Immunoprecipitation (see comments)
          Paraffin Sections (1-10 µg/ml)
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
          BackgroundP-glycoprotein is typically expressed in cells with secretory and excretory functions. Its highest expression is in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in various tumors, sarcomas and lymphomas.
          HostMouse
          Immunogen speciesHamster and Human
          ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
          CloneC219
          IsotypeIgG₁
          Specieshamster, human, mouse, rat
          Positive controlCell lines or frozen tissue, such as liver or colon
          FormLiquid
          FormulationIn PBS, 1% BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
          Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
          Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
          Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
          Citation
        • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
        • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
        • Application referencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.