PC345 Anti-Orexin A (14-33) (Ab-1) Rabbit pAb

PC345
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC345-1SET
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      Stocked 
      Discontinued
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      Available
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          Contact Customer Service

          Plastic ampoule 1 set
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          Description
          OverviewRecognizes orexin A in mouse and rat brain. Supplied with a control peptide.
          Catalogue NumberPC345
          Brand Family Calbiochem®
          Application Data
          Detection of human orexin A by staining frozen sections. Sample: Mouse brain tissue fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-1) Rabbit pAb (Cat. No. PC345) (2 µg/ml). Detection: DAB.
          References
          ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
          Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
          Sakurai, T., et al. 1998. Cell 92, 573.
          Schwartz, M.W. 1998. Nat. Med. 4, 385.
          Product Information
          FormLiquid
          Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide.
          Positive controlMouse or rat brain
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunofluorescence
          Application NotesELISA (see comments)
          Frozen Sections (1-3 µg/ml)
          Immunofluorescence (1-4 µg/ml)
          Application CommentsThis amino acid sequence is identical to orexin A from mouse, rat, and bovine. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with peptide at 10-6 M. Unconjugated control peptide has been included for use as an internal control for antibody specificity. This antibody has been reported to work for ELISA. This antibody is also available in serum form, Anti-Orexin A (Ab-2) Rabbit pAb (Cat.No. PC362). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsReconstitute peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Orexin A (14-33) (Ab-1) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Orexin A (14-33) (Ab-1) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC345

          References

          Reference overview
          De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
          Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
          Sakurai, T., et al. 1998. Cell 92, 573.
          Schwartz, M.W. 1998. Nat. Med. 4, 385.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-October-2007 RFH
          ApplicationELISA (see comments)
          Frozen Sections (1-3 µg/ml)
          Immunofluorescence (1-4 µg/ml)
          Application Data
          Detection of human orexin A by staining frozen sections. Sample: Mouse brain tissue fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-1) Rabbit pAb (Cat. No. PC345) (2 µg/ml). Detection: DAB.
          DescriptionPurified rabbit polyclonal antibody. Recognizes orexin A. Supplied with a control peptide.
          BackgroundThe hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
          IsotypeIgG
          Speciesbovine, human, mouse, rat
          Positive controlMouse or rat brain
          FormLiquid
          Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide (antibody only)
          CommentsThis amino acid sequence is identical to orexin A from mouse, rat, and bovine. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with peptide at 10-6 M. Unconjugated control peptide has been included for use as an internal control for antibody specificity. This antibody has been reported to work for ELISA. This antibody is also available in serum form, Anti-Orexin A (Ab-2) Rabbit pAb (Cat.No. PC362). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsReconstitute peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
          Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
          Sakurai, T., et al. 1998. Cell 92, 573.
          Schwartz, M.W. 1998. Nat. Med. 4, 385.