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MABN50 | Anti-Olig2 Antibody, clone 211F1.1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R WB, ICC, IHC, IP M Purified Monoclonal Antibody
      Catalogue NumberMABN50
      DescriptionAnti-Olig2 Antibody, clone 211F1.1
      Alternate Names
      • oligodendrocyte lineage transcription factor 2
      • oligodendrocyte-specific bHLH transcription factor 2
      • human protein kinase C-binding protein RACK17
      • Protein kinase C-binding protein 2
      • protein kinase C binding protein 2
      • Class B basic helix-loop-helix protein 1
      • Protein kinase C-binding protein RACK17
      • basic domain, helix-loop-helix protein, class B, 1
      Background InformationOligodendrocyte lineage transcription factor 2 (Olig2) is a protein belonging to group A of the OLIG family of basic helix-loop-helix transcription factors (bHLH). bHLH proteins are divided into 6 groups (A-F), and characterized by their biochemical properties and structure. Olig2 is classified as group A due to its ability to form heterodimers with other bHLH proteins and binding to the E box. Olig2 has a crucial role during development in specifying the final location of motor neurons and oligodendrocytes in the spinal cord, along with the development within the hindbrain of somatic motor neurons. Olig2 expression is observed within oligodendrocytes and in developing astrocytes. Recent studies have shown strong expression of Olig2 in human oligodendrogliomas, primary tumors within the central nervous system, and moderate expression in astrocytomas. These observations have led to the proposal of Olig2 having potential as an oligodendroglioma diagnostic marker.
      Product Information
      • Mouse brain lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect Olig2 using this Anti-Olig2 Antibody, clone 211F1.1 validated for use in WB, IC, IH & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: This antibody has been shown to detect Olig2 in coronal brain tissue as reported by an independent laboratory (Cai, J., et al. (2007). Development. 134:1887-1899).

      Immunocytochemistry Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).

      Immunoprecipitation Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Olig2.
      Epitope Unknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Olig2.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human and rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OLIG2
      • OLIGO2
      • RACK17
      • PRKCBP2
      • Oligo2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).

      SIZE: 323 amino acids; 32385 Da

      SUBUNIT: Interacts with NKX2-2 (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2 (By similarity).

      TISSUE SPECIFICITY: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

      DOMAIN: The bHLH is essential for interaction with NKX2-2 (By similarity).DISEASE:SwissProt: Q13516 # A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.

      SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight~37 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Olig2 on 10 µg of mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Olig2, clone 211F1.1 - 2146004 2146004
      Anti-Olig2, clone 211F1.1 - 2358873 2358873
      Anti-Olig2, clone 211F1.1 - 2388987 2388987
      Anti-Olig2, clone 211F1.1 - 2445062 2445062
      Anti-Olig2, clone 211F1.1 - 2445354 2445354
      Anti-Olig2, clone 211F1.1 - 1969809 1969809
      Anti-Olig2, clone 211F1.1 - 1994918 1994918
      Anti-Olig2, clone 211F1.1 - 2026416 2026416
      Anti-Olig2, clone 211F1.1 - 2049816 2049816
      Anti-Olig2, clone 211F1.1 - 2070427 2070427
      Anti-Olig2, clone 211F1.1 - 2111397 2111397
      Anti-Olig2, clone 211F1.1 - 2228141 2228141
      Anti-Olig2, clone 211F1.1 - 2299163 2299163
      Anti-Olig2, clone 211F1.1 - 2522249 2522249
      Anti-Olig2, clone 211F1.1 - JBC1791558 JBC1791558
      Anti-Olig2, clone 211F1.1 - NG1820263 NG1820263
      Anti-Olig2, clone 211F1.1 - NG1852660 NG1852660
      Anti-Olig2, clone 211F1.1 - NG1900694 NG1900694
      Anti-Olig2, clone 211F1.1 - NRG1759339 NRG1759339
      Anti-Olig2, clone 211F1.1 -2491257 2491257
      Anti-Olig2, clone 211F1.1 -2552644 2552644
      Anti-Olig2, clone 211F1.1 -2575745 2575745
      Anti-Olig2, clone 211F1.1 -2594342 2594342
      Anti-Olig2, clone 211F1.1 -2649639 2649639
      Anti-Olig2, clone 211F1.1 -2667338 2667338
      Anti-Olig2, clone 211F1.1 -2701029 2701029
      Anti-Olig2, clone 211F1.1 -2730966 2730966
      Anti-Olig2, clone 211F1.1 -2780489 2780489

      References | 15 Available | See All References

      Reference overviewApplicationPub Med ID
      Protein carbonylation after traumatic brain injury: cell specificity, regional susceptibility, and gender differences.
      Lazarus, RC; Buonora, JE; Jacobowitz, DM; Mueller, GP
      Free radical biology & medicine 78 89-100 2015

      Show Abstract
      25462645 25462645
      Tis21 is required for adult neurogenesis in the subventricular zone and for olfactory behavior regulating cyclins, BMP4, Hes1/5 and Ids.
      Farioli-Vecchioli, S; Ceccarelli, M; Saraulli, D; Micheli, L; Cannas, S; D'Alessandro, F; Scardigli, R; Leonardi, L; Cinà, I; Costanzi, M; Mattera, A; Cestari, V; Tirone, F
      Frontiers in cellular neuroscience 8 98 2014

      Show Abstract
      24744701 24744701
      Restoration of oligodendrocyte pools in a mouse model of chronic cerebral hypoperfusion.
      McQueen, J; Reimer, MM; Holland, PR; Manso, Y; McLaughlin, M; Fowler, JH; Horsburgh, K
      PloS one 9 e87227 2014

      Show Abstract
      24498301 24498301
      Diverse behaviors of outer radial glia in developing ferret and human cortex.
      Gertz, CC; Lui, JH; LaMonica, BE; Wang, X; Kriegstein, AR
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 2559-70 2014

      Show Abstract
      24523546 24523546
      Calreticulin and other components of endoplasmic reticulum stress in rat and human inflammatory demyelination.
      Ní Fhlathartaigh, M; McMahon, J; Reynolds, R; Connolly, D; Higgins, E; Counihan, T; Fitzgerald, U
      Acta neuropathologica communications 1 37 2013

      Show Abstract
      Immunohistochemistry24252779 24252779
      Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth.
      Verginelli, F; Perin, A; Dali, R; Fung, KH; Lo, R; Longatti, P; Guiot, MC; Del Maestro, RF; Rossi, S; di Porzio, U; Stechishin, O; Weiss, S; Stifani, S
      Nature communications 4 2956 2013

      Show Abstract
      24356439 24356439
      RING finger protein 11 (RNF11) modulates susceptibility to 6-OHDA-induced nigral degeneration and behavioral deficits through NF-κB signaling in dopaminergic cells.
      Pranski, EL; Dalal, NV; Sanford, CV; Herskowitz, JH; Gearing, M; Lazo, C; Miller, GW; Lah, JJ; Levey, AI; Betarbet, RS
      Neurobiology of disease 54 264-79 2013

      Show Abstract
      23318928 23318928
      Prion replication elicits cytopathic changes in differentiated neurosphere cultures.
      Iwamaru, Y; Takenouchi, T; Imamura, M; Shimizu, Y; Miyazawa, K; Mohri, S; Yokoyama, T; Kitani, H
      Journal of virology 87 8745-55 2013

      Show Abstract
      23740992 23740992
      Synergistic binding of transcription factors to cell-specific enhancers programs motor neuron identity.
      Mazzoni, EO; Mahony, S; Closser, M; Morrison, CA; Nedelec, S; Williams, DJ; An, D; Gifford, DK; Wichterle, H
      Nature neuroscience 16 1219-27 2013

      Show Abstract
      Immunocytochemistry23872598 23872598
      Transgenic overexpression of Sox17 promotes oligodendrocyte development and attenuates demyelination.
      Ming, X; Chew, LJ; Gallo, V
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 12528-42 2013

      Show Abstract
      Immunohistochemistry23884956 23884956

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