A part of MilliporeSigma

AB5991 | Anti-Olig1 Antibody

AB5991
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5991
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Olig1 Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • Oligodendrocyte culture, Brain
      PresentationAffinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Applications
      ApplicationAnti-Olig1 Antibody detects level of Olig1 & has been published & validated for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 77-91 of Olig1.
      HostRabbit
      SpecificityRecognizes Olig1. The molecular weight of the protein is 25,430 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OLIG1
      • BHLHB6
      • Oligo1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8TAK6 # Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube (By similarity).
      SIZE: 271 amino acids; 27905 Da
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      TISSUE SPECIFICITY: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.
      SIMILARITY: SwissProt: Q8TAK6 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight25.4 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-OLIG1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2120031 2120031
      RABBIT ANTI-OLIG1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2398854 2398854
      RABBIT ANTI-OLIG1 -2605418 2605418
      RABBIT ANTI-OLIG1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2201395 2201395

      References

      Reference overviewPub Med ID
      Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.
      Xue, Yuanchao, et al.
      Cell, 152: 82-96 (2013) 2013

      Show Abstract
      23313552 23313552
      Regulation of glioblastoma multiforme stem-like cells by inhibitor of DNA binding proteins and oligodendroglial lineage-associated transcription factors.
      Wu, Y; Richard, JP; Wang, SD; Rath, P; Laterra, J; Xia, S
      Cancer science 103 1028-37 2012

      Show Abstract
      22380883 22380883

      Data Sheet

      Title
      RABBIT ANTI-OLIG1 AFFINITY PURIFIED POLYCLONAL ANTIBODY

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB15620 Anti-Olig1 Antibody Show Pricing & Availability
      MAB5540 Anti-Olig1 Antibody Show Pricing & Availability

      Product Families

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies