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MABE1039 | Anti-Nup96 Antibody, clone 4H5

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB, ICC R Purified Monoclonal Antibody
      Catalogue NumberMABE1039
      DescriptionAnti-Nup96 Antibody, clone 4H5
      Alternate Names
      • Nuclear pore complex protein Nup98-Nup96
      • Nuclear pore complex protein Nup98
      • 98 kDa nucleoporin
      • Nucleoporin Nup98
      • Nup98
      • Nuclear pore complex protein Nup96
      • 96 kDa nucleoporin
      • Nucleoporin Nup96
      • Nup96
      • Nup96
      Background InformationThe murine gene Nup98 (Gene ID 269966; also known as AI849286, 4732457F17) encodes the 1816-amino acid nuclear pore complex protein Nup98-Nup96 (UniProt Q6PFD9), which is posttranslationally autocleaved between aa 800 and 801 to form Nup98 (aa 1-800) and Nup96 (aa 801-1816). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. The core structure of the NPC is maintained by the Nup93-205 complex sandwiched between the supporting structures of the Nup107-160 complex on the nuclear and cytoplasmic faces of the pore. Nup96 is a component of the Nup107-160 complex, the largest subunit of the NPC. Nup96 knockout is reported to be fatal in mice and Nup96(+/-) mice with low levels of Nup96 display impaired immune response and are highly susceptible to viral infection.
      Product Information
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
      Immunocytochemistry Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse Nup96 near the N-terminus.
      EpitopeNear N-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityReacts with Nup98-Nup96 precursor (isoforms 1, 2, and 6), nucleoporin Nup96 (isoform 5), but not nucleoporin Nup98 (isoform 3).
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nup98
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~120 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nup96 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information