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ABE1387 | Anti-Nuclear factor 1/C Antibody

ABE1387
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, ChIP, EMSA Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABE1387
      DescriptionAnti-Nuclear factor 1/C Antibody
      Alternate Names
      • Nuclear factor 1 C-type
      • NF1-C
      • CCAAT-box-binding transcription factor
      • CCAAT-box-binding transcription factor
      • CTF
      • Nuclear factor I/C
      • NF-I/C
      • NFI-C
      • TGGCA-binding protein
      Background InformationThrough evolution, eukaryotic cells have incorporated numerous viral promoter elements and incorporated them as part of their own gene regulatory elements. Nuclear factor 1/C was originally recognized as a transcription factor that bound the origin of replication for adenovirus type 2. Nuclear factor 1/C belongs to the CTF/NF-I family of eukaryotic DNA replication and transcription factors and is important in regulating the expression of many cellular developmental systems such as that of tooth development as well as multiple TGF-beta1 signaling mediated pathways systems.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Nuclear factor 1/C Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of Nuclear factor 1/C.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Electrophoretic Mobility Shift Assay
      Application NotesChromatin Immunoprecipitation Assay (ChIP): A representative lot of this antibody has been reported to work in ChIP. See Hebbar, P.B., and Archer, T.K. (2007), Vicent G.P. et al. (2010), and Brun, M., et al. (2013).

      Electrophoretic Mobility Shift Assay (EMSA): A representative lot of this antibody has been reported to work in EMSA. See Johansson E.M., et al. (2003) and Kannius-Janson, M., et al. (2002).
      Biological Information
      ImmunogenRecombinant protein corresponding to full length Human NFIC.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes multiple isoforms of Nuclear factor 1/C
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NFIC
      • NFI
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~45-55 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U251 cell lysate.

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected NFIC isoforms at ~45-55 kDa in a U251 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nuclear factor 1/C - Q2459142 Q2459142

      References

      Reference overviewApplicationPub Med ID
      Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.
      Brun, M; Glubrecht, DD; Baksh, S; Godbout, R
      The Journal of biological chemistry 288 24104-15 2013

      Show Abstract
      Western Blotting23839947 23839947
      Chromatin-dependent cooperativity between site-specific transcription factors in vivo.
      Hebbar, Pratibha B and Archer, Trevor K
      J. Biol. Chem., 282: 8284-91 (2007) 2007

      Show Abstract
      17186943 17186943
      The p53 tumor suppressor gene is regulated in vivo by nuclear factor 1-C2 in the mouse mammary gland during pregnancy.
      Johansson, Eva M, et al.
      Oncogene, 22: 6061-70 (2003) 2003

      Show Abstract
      12955085 12955085
      Nuclear factor 1-C2 contributes to the tissue-specific activation of a milk protein gene in the differentiating mammary gland.
      Kannius-Janson, Marie, et al.
      J. Biol. Chem., 277: 17589-96 (2002) 2002

      Show Abstract
      11877413 11877413