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07-218 | Anti-Notch1 Antibody, extracellular domain

07-218
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-218
      Replaces04-1046
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Notch1 Antibody, extracellular domain
      Background InformationThe Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems. This family of proteins function both as membrane cell receptors and as transcription factors. Activation of Notch by cell-cell interactions causes a transcription inhibitory effect that enables inhibition of differentiation in some cells but not in others. As a consequence, some cells adopt a particular fate while other progenitors remain uncommitted. The Notch protein is important in cell fate during myogenesis, neurogenesis, oogenesis, and wing and eye development in Drosophila. In mammals, four Notch genes were identified (Notch 1-4) that are expressed in a wide variety of cells and play a crucial role in differentiation and development.
      References
      Product Information
      FormatPurified
      Control
      • L/N17 transfected Notch1 cell lysate
      PresentationProtein A purified immunoglobulin presented in 0.1M Tris-Glycine, 0.15M NaCl, and 0.05% Sodium Azide, pH 7.4
      Applications
      ApplicationAnti-Notch1 Antibody, extracellular domain is a high quality Rabbit Polyclonal Antibody for the detection of Notch1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenN-terminal GST fusion protein corresponding to residues 381-853 of rat Notch1
      HostRabbit
      SpecificityNotch1
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteUsed in mouse in the following publication: Gulino, R., et al, European Journal of Neuroscience, Vol. 31, pp. 1423–1434, 2010.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
      Gene Symbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
      SIZE: 2556 amino acids; 272500 Da
      SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).
      TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
      DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.
      SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
      Molecular Weight260kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from L/N17 rat Notch1 transfected fibroblast cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Notch1, extracellular domain 2487903
      Anti-Notch1, extracellular domain - 0609040500 0609040500
      Anti-Notch1, extracellular domain - 20442 20442
      Anti-Notch1, extracellular domain - DAM1745314 DAM1745314
      Anti-Notch1, extracellular domain - JBC1888235 JBC1888235
      Anti-Notch1, extracellular domain -2604223 2604223
      Anti-Notch1, extracellular domain -2644562 2644562
      Anti-Notch1, extracellular domain -2733572 2733572

      References | 11 Available | See All References

      Reference overviewApplicationPub Med ID
      Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease.
      Gulino, R; Parenti, R; Gulisano, M
      BioMed research international 2015 654637 2015

      Show Abstract
      26064939 26064939
      Involvement of brain-derived neurotrophic factor and sonic hedgehog in the spinal cord plasticity after neurotoxic partial removal of lumbar motoneurons.
      Rosario Gulino,Massimo Gulisano
      Neuroscience research 73 2012

      Show Abstract
      22579680 22579680
      Methylseleninic acid enhances paclitaxel efficacy for the treatment of triple-negative breast cancer.
      Qi, Y; Fu, X; Xiong, Z; Zhang, H; Hill, SM; Rowan, BG; Dong, Y
      PloS one 7 e31539 2012

      Show Abstract
      22348099 22348099
      Expression of cell fate determinants and plastic changes after neurotoxic lesion of adult mice spinal cord by cholera toxin-B saporin.
      Gulino, Rosario, et al.
      Eur. J. Neurosci., 31: 1423-34 (2010) 2010

      Show Abstract
      20384775 20384775
      The human acid alpha-glucosidase gene is a novel target of the Notch-1/Hes-1 signaling pathway
      Yan, B., et al
      J Biol Chem, 277:29760-4 (2002) 2002

      Immunoblotting (Western)12065598 12065598
      Nuclear access and action of notch in vivo
      Struhl, G and Adachi, A
      Cell, 93:649-60 (1998) 1998

      9604939 9604939
      Notch signaling: direct or what?
      Weinmaster, G
      Curr Opin Genet Dev, 8:436-42 (1998) 1998

      9729720 9729720
      Delta-1 activation of notch-1 signaling results in HES-1 transactivation
      Jarriault, S, et al
      Mol Cell Biol, 18:7423-31 (1998) 1998

      9819428 9819428
      NF-kappaB2 is a putative target gene of activated Notch-1 via RBP-Jkappa
      Oswald, F, et al
      Mol Cell Biol, 18:2077-88 (1998) 1998

      9528780 9528780
      Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane
      Blaumueller, C M, et al
      Cell, 90:281-91 (1997) 1997

      9244302 9244302

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies