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07-189 | Anti-Notch 4 Antibody

07-189
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-189
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Notch 4 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
      Applications
      ApplicationAnti-Notch 4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Notch 4 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tag fusion protein containing the carboxy-terminus of murine Notch 4
      HostRabbit
      Specificitytruncated, intracellular domain of Int3/Notch 4, possibly full-length Notch 4; does not cross-react with Notch 1, 2 or 3
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined.
      Gene Symbol
      • NOTCH4
      • INT3
      • MGC74442
      • OTTHUMP00000062678
      • NOTCH3
      • hNotch4
      • notch4
      • FLJ16302
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99466 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).
      SIZE: 2003 amino acids; 209622 Da
      SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 4 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
      TISSUE SPECIFICITY: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
      SIMILARITY: SwissProt: Q99466 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 28 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
      Molecular Weight68kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from 293 cells transfected with the intracellular domain of murine Notch 4
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Notch 4 (rabbit polyclonal IgG) - 2428459 2428459
      Anti-Notch 4 (rabbit polyclonal IgG) - 2318930 2318930
      Anti-Notch 4 - 20370 20370
      Anti-Notch 4 - 29748 29748
      Anti-Notch 4 - DAM1467521 DAM1467521
      Anti-Notch 4 - DAM1641049 DAM1641049
      Anti-Notch 4 -2620130 2620130

      References

      Reference overviewApplicationPub Med ID
      Cripto-1 as a novel therapeutic target for triple negative breast cancer.
      Castro, NP; Fedorova-Abrams, ND; Merchant, AS; Rangel, MC; Nagaoka, T; Karasawa, H; Klauzinska, M; Hewitt, SM; Biswas, K; Sharan, SK; Salomon, DS
      Oncotarget 6 11910-29 2015

      Show Abstract
      26059540 26059540
      Tumor-suppressive activity of Lunatic Fringe in prostate through differential modulation of Notch receptor activation.
      Zhang, S; Chung, WC; Wu, G; Egan, SE; Xu, K
      Neoplasia (New York, N.Y.) 16 158-67 2014

      Show Abstract
      Western Blotting24709423 24709423
      Genome-wide reprogramming of the chromatin landscape underlies endocrine therapy resistance in breast cancer.
      Magnani, L; Stoeck, A; Zhang, X; Lánczky, A; Mirabella, AC; Wang, TL; Gyorffy, B; Lupien, M
      Proceedings of the National Academy of Sciences of the United States of America 110 E1490-9 2013

      Show Abstract
      23576735 23576735
      Notch4 normalization reduces blood vessel size in arteriovenous malformations.
      Murphy, PA; Kim, TN; Lu, G; Bollen, AW; Schaffer, CB; Wang, RA
      Science translational medicine 4 117ra8 2012

      Show Abstract
      22261032 22261032
      Elongin C is a mediator of Notch4 activity in human renal tubule cells.
      Cummins, TD; Mendenhall, MD; Lowry, MN; Korte, EA; Barati, MT; Khundmiri, SJ; Salyer, SA; Klein, JB; Powell, DW
      Biochimica et biophysica acta 1814 1748-57 2011

      Show Abstract
      Western Blotting22001063 22001063
      Transforming acidic coiled-coil protein-3 (Tacc3) acts as a negative regulator of Notch signaling through binding to CDC10/Ankyrin repeats.
      Sharon Bargo,Ahmed Raafat,David McCurdy,Idean Amirjazil,Youmin Shu,June Traicoff,Joshua Plant,Barbara K Vonderhaar,Robert Callahan
      Biochemical and biophysical research communications 400 2010

      Show Abstract Full Text Article
      20804727 20804727
      Glucocorticoid and growth factor synergism requirement for Notch4 chromatin domain activation.
      Wu, J; Bresnick, EH
      Molecular and cellular biology 27 2411-22 2007

      Show Abstract
      Western Blotting17220278 17220278