A part of MilliporeSigma

MAB5414 | Anti-Notch 1 Antibody, extracellular, clone 8G10

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R FC, WB, IHC SHm Culture Supernatant Monoclonal Antibody
      Catalogue NumberMAB5414
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Notch 1 Antibody, extracellular, clone 8G10
      Alternate Names
      • Motch A
      • mT14
      • p300
      Background InformationNotch 1 is a type 1 membrane protein that serves as a receptor for the membrane bound ligands jagged-1 and -2 and delta-1 to regulate cell fate determination through the implementation of differentiation, proliferation and apoptotic programs. Upon ligand binding, the Notch intracellular domain (NICD) is released by proteolytic processing and subsequently translocates to the nucleus where it forms a transcriptionally active complex with RBP-J kappa to activate genes of the enhancer of split locus. Notch-1 may also be involved in cell specification and/or differentiation and body segment determination in the postimplantation embryo. In the adult animal, Notch-1 is highly expressed in the brain, lung, and thymus with lower expression levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart.
      Product Information
      FormatCulture Supernatant
      • POSITIVE CONTROL: brain, lung, thymus.
      PresentationUnpurified tissue culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at final concentration of 10 μg/mL.
      ApplicationUse Anti-Notch 1 Antibody, extracellular, clone 8G10 (Syrian Hamster Monoclonal Antibody) validated in FC, WB, IHC to detect Notch 1 also known as Motch A, mT14, p300.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot

      Immunohistochemistry on paraformaldehyde fixed frozen tissue sections.

      Flow Cytometry

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA GST fusion protein comprising amino acids 1299-1492 of mouse Notch 1
      HostSyrian Hamster
      SpecificityReacts with natural and recombinant Notch1. Recognizes a band migrating at approximately 300 kDa on Western blots of rat and mouse protein extracts. A cross reactive band migrating at approximately 230 kDa is observed in some samples and is believed to correspond to the processed extracellular fragment of Notch 1.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
      Gene Symbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
      SIZE: 2556 amino acids; 272500 Da
      SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).
      TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
      DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.
      SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Notch 1, extracellular, clone 8G10 - 2037552 2037552
      Anti-Notch 1, extracellular, clone 8G10 - 2165841 2165841
      Anti-Notch 1, extracellular, clone 8G10 - LV1761054 LV1761054
      Anti-Notch 1, extracellular, clone 8G10 -2608423 2608423


      Reference overviewApplicationPub Med ID
      Distinct TCR signaling pathways drive proliferation and cytokine production in T cells.
      Guy, CS; Vignali, KM; Temirov, J; Bettini, ML; Overacre, AE; Smeltzer, M; Zhang, H; Huppa, JB; Tsai, YH; Lobry, C; Xie, J; Dempsey, PJ; Crawford, HC; Aifantis, I; Davis, MM; Vignali, DA
      Nature immunology 14 262-70 2013

      Show Abstract
      23377202 23377202
      Impaired angiogenesis and altered Notch signaling in mice overexpressing endothelial Egfl7.
      Nichol, D; Shawber, C; Fitch, MJ; Bambino, K; Sharma, A; Kitajewski, J; Stuhlmann, H
      Blood 116 6133-43 2010

      Show Abstract
      Western Blotting20947685 20947685
      Notch in vertebrates
      Robey, E.
      Curr. Opin. Genet. Dev., 7:551-557 (1997) 1997

      9309189 9309189

      Data Sheet

      Anti-Notch 1, extracellular, clone 8G10 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies