A part of MilliporeSigma

MAB5460 | Anti-Nkx2.1 Antibody, clone 8G7-G3-1

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Ascites Monoclonal Antibody
      Catalogue NumberMAB5460
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nkx2.1 Antibody, clone 8G7-G3-1
      Alternate Names
      • TTF-1
      • Thyroid Transcription Factor-1
      Product Information
      PresentationAscites fluid. Liquid. Contains no preservative.
      ApplicationThis Anti-Nkx2.1 Antibody is validated for use in WB, IH(P) for the detection of Nkx2.1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot. The antibody reacts with a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the Nkx2.1 positive cell lines MLE15, H441-4, H345 and rat type II pmeumocyte cells.

      Immunohistochemistry. The antibody can be used on formalin-fixed paraffin embedded tissue. Heat induced epitope retrieval is recommended.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant rat Nkx2.1.
      SpecificityNkx2.1 (Thyroid Transcription Factor-1, TTF-1).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NKX2-1
      • BCH
      • TEBP
      • TITF1
      • TTF1
      • NKX2.1
      • NK-2
      • TTF-1
      • BHC
      • NKX2A
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43699 # Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
      SIZE: 371 amino acids; 38596 Da
      TISSUE SPECIFICITY: Thyroid and lung.
      PTM: Phosphorylated on serine residues (By similarity).
      DISEASE: SwissProt: P43699 # Defects in TITF1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. & Defects in TITF1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
      SIMILARITY: SwissProt: P43699 ## Belongs to the NK-2 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MAB5460 - 2450195 2450195
      MAB5460 - 2279543 2279543
      MOUSE ANTI-NKX2.1 - 2510329 2510329
      MOUSE ANTI-NKX2.1 -2697593 2697593
      MOUSE ANTI-NKX2.1 -2710263 2710263
      MOUSE ANTI-NKX2.1 -2822049 2822049
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY - 2123647 2123647
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY - 2148885 2148885
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY - 2015976 2015976
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY - 2025988 2025988
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY - 2041570 2041570
      MOUSE ANTI-NKX2.1 MONOCLONAL ANTIBODY -2643526 2643526


      Reference overviewApplicationPub Med ID
      Grainyhead-like 2 (GRHL2) distribution reveals novel pathophysiological differences between human idiopathic pulmonary fibrosis and mouse models of pulmonary fibrosis.
      Varma, S; Mahavadi, P; Sasikumar, S; Cushing, L; Hyland, T; Rosser, AE; Riccardi, D; Lu, J; Kalin, TV; Kalinichenko, VV; Guenther, A; Ramirez, MI; Pardo, A; Selman, M; Warburton, D
      American journal of physiology. Lung cellular and molecular physiology 306 L405-19 2014

      Show Abstract
      24375798 24375798
      Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
      Liu, Y; Weick, JP; Liu, H; Krencik, R; Zhang, X; Ma, L; Zhou, GM; Ayala, M; Zhang, SC
      Nature biotechnology 31 440-7 2013

      Show Abstract
      23604284 23604284
      Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells.
      Liu, Y; Liu, H; Sauvey, C; Yao, L; Zarnowska, ED; Zhang, SC
      Nature protocols 8 1670-9 2013

      Show Abstract
      Immunocytochemistry23928500 23928500
      The specification of telencephalic glutamatergic neurons from human pluripotent stem cells.
      Boisvert, Erin M, et al.
      J Vis Exp, (2013) 2013

      Show Abstract
      23603787 23603787

      Data Sheet

      Anti-Nkx2.1 - Data Sheet

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