A part of MilliporeSigma

AB5983 | Anti-Nkx-3.1 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB5983
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nkx-3.1 Antibody
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      ApplicationAnti-Nkx-3.1 Antibody is an antibody against Nkx-3.1 for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunohistochemistry: 1:200-1:1,000.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 70-83 of Nkx3.1.
      SpecificityRecognizes Nkx3.1. The molecular weight of the protein is 21,623 Daltons (v2 21,097, v3 19,077, v4 18,449).
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3A protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.[supplied by OMIM]
      Gene Symbol
      • NKX3-1
      • NKX3A
      • NKX3.1
      • BAPX2
      • NKX3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99801 # Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Could play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate.
      SIZE: 234 amino acids; 26350 Da
      SUBUNIT: Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF.
      TISSUE SPECIFICITY: Highly expressed in the prostate and, at a lower level, in the testis.
      DISEASE:SwissProt: Q99801 # NKX3-1 has been thought to be one of the target gene of the 8p21 loss of heterozygosity, common in prostate cancer, but neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.
      SIMILARITY: SwissProt: Q99801 ## Belongs to the NK-3 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.


      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information