05-233 Anti-Nitrotyrosine Antibody, clone 1A6

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt ICC, IHC, IP, WB M Purified Monoclonal Antibody
      Catalogue Number05-233
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nitrotyrosine Antibody, clone 1A6
      Background InformationNitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
      Product Information
      • Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
        Immunoblotting: Nitrated-BSA
        Indirect ELISA: nitrated-BSA
        Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
      ApplicationAnti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenNitrated KLH
      SpecificityRecognizes nitrated protein.
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightPredicted MW for nitrated-BSA: 66 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nitrotyrosine, clone 1A6 - 21380052138005
      Anti-Nitrotyrosine, clone 1A6 - 24304792430479
      Anti-Nitrotyrosine, clone 1A6 - 24557192455719
      Anti-Nitrotyrosine, clone 1A6 - 1559015590
      Anti-Nitrotyrosine, clone 1A6 - 1668516685
      Anti-Nitrotyrosine, clone 1A6 - 1846418464
      Anti-Nitrotyrosine, clone 1A6 - 1876018760
      Anti-Nitrotyrosine, clone 1A6 - 1945419454
      Anti-Nitrotyrosine, clone 1A6 - 19581141958114
      Anti-Nitrotyrosine, clone 1A6 - 19996801999680


      Reference overviewApplicationSpeciesPub Med ID
      Ω3 Supplementation and intermittent hypobaric hypoxia induce cardioprotection enhancing antioxidant mechanisms in adult rats.
      Herrera, EA; Farías, JG; González-Candia, A; Short, SE; Carrasco-Pozo, C; Castillo, RL
      Marine drugs  13  838-60  2015

      Show Abstract
      25658050 25658050
      Myofibrillar Ca(2+) sensitivity is uncoupled from troponin I phosphorylation in hypertrophic obstructive cardiomyopathy due to abnormal troponin T.
      Bayliss, CR; Jacques, AM; Leung, MC; Ward, DG; Redwood, CS; Gallon, CE; Copeland, O; McKenna, WJ; Dos Remedios, C; Marston, SB; Messer, AE
      Cardiovascular research  97  500-8  2013

      Show Abstract
      23097574 23097574
      Increased microparticle production and impaired microvascular endothelial function in aldosterone-salt-treated rats: protective effects of polyphenols.
      López Andrés, N; Tesse, A; Regnault, V; Louis, H; Cattan, V; Thornton, SN; Labat, C; Kakou, A; Tual-Chalot, S; Faure, S; Challande, P; Osborne-Pellegrin, M; Martínez, MC; Lacolley, P; Andriantsitohaina, R
      PloS one  7  e39235  2012

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      22808030 22808030
      Arginine and asymmetric dimethylarginine in puromycin aminonucleoside-induced chronic kidney disease in the rat.
      Chen, GF; Moningka, NC; Sasser, JM; Zharikov, S; Cunningham, M; Tain, YL; Schwartz, IF; Baylis, C
      American journal of nephrology  35  40-8  2012

      Show Abstract
      22179117 22179117
      Nebivolol does not protect against 5/6 ablation/infarction induced chronic kidney disease in rats - comparison with angiotensin II receptor blockade.
      Sasser, JM; Moningka, NC; Tsarova, T; Baylis, C
      Life sciences  91  54-63  2012

      Show Abstract
      Western Blotting22727796 22727796
      Local oxidative stress expansion through endothelial cells--a key role for gap junction intercellular communication.
      Feine, I; Pinkas, I; Salomon, Y; Scherz, A
      PloS one  7  e41633  2012

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      22911831 22911831
      Role of nitric oxide synthases in elastase-induced emphysema.
      Boyer, L; Plantier, L; Dagouassat, M; Lanone, S; Goven, D; Caramelle, P; Berrehar, F; Kerbrat, S; Dinh-Xuan, AT; Crestani, B; Le Gouvello, S; Boczkowski, J
      Laboratory investigation; a journal of technical methods and pathology  91  353-62  2011

      Show Abstract
      20956973 20956973
      Butyrylcholinesterase inhibitors ameliorate cognitive dysfunction induced by amyloid-β peptide in mice.
      Yoko Furukawa-Hibi,Tursun Alkam,Atsumi Nitta,Akihiro Matsuyama,Hiroyuki Mizoguchi,Kazuhiko Suzuki,Saliha Moussaoui,Qian-Sheng Yu,Nigel H Greig,Taku Nagai,Kiyofumi Yamada
      Behavioural brain research  225  2011

      Show Abstract
      21820013 21820013
      Microparticles from apoptotic monocytes enhance nitrosative stress in human endothelial cells.
      Mastronardi, Maria Letizia, et al.
      Fundam Clin Pharmacol, 25: 653-60 (2011)  2011

      Show Abstract
      21105910 21105910
      Analysis of the mitochondrial proteome in multiple sclerosis cortex.
      Broadwater, L; Pandit, A; Clements, R; Azzam, S; Vadnal, J; Sulak, M; Yong, VW; Freeman, EJ; Gregory, RB; McDonough, J
      Biochimica et biophysica acta  1812  630-41  2011

      Show Abstract
      21295140 21295140


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies