482728 | Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb

482728
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      482728-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~130 kDa iNOS protein.
          Catalogue Number482728
          Brand Family Calbiochem®
          SynonymsAnti-iNOS
          Application Data
          Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
          References
          ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
          Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
          Bredt, D.S., et al. 1991. Nature 351, 714.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive controlRaw 264.7 cells treated with IFNγ and LPS
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesFrozen Sections (1:500)
          Immunoblotting (1:2000)
          Immunocytochemistry (1:500)
          Paraffin Sections (1:500)
          Application CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

          Recommended Procedure for Immunocytochemistry of Anti-iNOS:
          1. Grow cells in appropriate medium.
          2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
          3. Wash cells 3-5 min with phosphate buffered saline (PBS).
          4. Permeabilize with 2% saponin for 10 min at room temperature.
          5. Fix in 4% formalin for 10 min at room temperature.
          6. Wash 3-5 min with PBS.
          7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
          8. Wash 3-5 min with PBS.
          9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
          10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
          Biological Information
          Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
          ImmunogenMouse
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          482728

          References

          Reference overview
          Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
          Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
          Bredt, D.S., et al. 1991. Nature 351, 714.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2007 RFH
          SynonymsAnti-iNOS
          ApplicationFrozen Sections (1:500)
          Immunoblotting (1:2000)
          Immunocytochemistry (1:500)
          Paraffin Sections (1:500)
          Application Data
          Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
          HostRabbit
          Immunogen speciesMouse
          Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlRaw 264.7 cells treated with IFNγ and LPS
          FormLiquid
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

          Recommended Procedure for Immunocytochemistry of Anti-iNOS:
          1. Grow cells in appropriate medium.
          2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
          3. Wash cells 3-5 min with phosphate buffered saline (PBS).
          4. Permeabilize with 2% saponin for 10 min at room temperature.
          5. Fix in 4% formalin for 10 min at room temperature.
          6. Wash 3-5 min with PBS.
          7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
          8. Wash 3-5 min with PBS.
          9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
          10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
          Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
          Bredt, D.S., et al. 1991. Nature 351, 714.