A part of MilliporeSigma

MAB1886 | Anti-Nidogen Antibody, G1 domain, clone JF6

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ELISA, WB, FUNC R Purified Monoclonal Antibody
      Catalogue NumberMAB1886
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nidogen Antibody, G1 domain, clone JF6
      Alternate Names
      • Entactin
      Product Information
      PresentationLiquid at 1 mg/mL in 0.02 MPB + 0.25 M NaCl, containing 0.1% sodium azide as preservative. pH 7.6
      ApplicationDetect Nidogen using this Anti-Nidogen Antibody, G1 domain, clone JF6 validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      • Affects Function
      Application NotesWestern blot: Recognizes native and denatured nidogen.

      This antibody is able to block the binding of nidogen to laminin.


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fragment corresponding to the G1 region of mouse nidogen.
      EpitopeG1 domain
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.
      Gene Symbol
      • NID1
      • enactin
      • NID
      • Entactin
      • entactin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14543 # Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.
      SIZE: 1247 amino acids; 136453 Da
      SUBUNIT: Interacts with FBLN1 and LGALS3BP (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane.
      PTM: N- and O-glycosylated.
      SIMILARITY: SwissProt: P14543 ## Contains 6 EGF-like domains. & Contains 4 LDL-receptor class B repeats. & Contains 1 NIDO domain. & Contains 1 nidogen G2 beta-barrel domain. & Contains 1 thyroglobulin type-1 domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RAT ANTI-NIDOGEN [ENTACTIN] G2 DOMAIN -2807737 2807737


      Reference overviewApplicationSpeciesPub Med ID
      The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens.
      Behrens, DT; Villone, D; Koch, M; Brunner, G; Sorokin, L; Robenek, H; Bruckner-Tuderman, L; Bruckner, P; Hansen, U
      The Journal of biological chemistry 287 18700-9 2012

      Show Abstract
      22493504 22493504
      Human amniotic membrane, like corneal epithelial basement membrane, manifests the alpha5 chain of type IV collagen
      Endo, Ken-ichi, et al
      Invest Ophthalmol Vis Sci, 45:1771-4 (2004) 2004

      Immunoblotting (Western)Human15161839 15161839
      Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies
      Ries, A, et al
      Eur J Biochem, 268:5119-28 (2001) 2001

      11589703 11589703
      Rescue of mammary epithelial cell apoptosis and entactin degradation by a tissue inhibitor of metalloproteinases-1 transgene.
      Alexander, C M, et al.
      J. Cell Biol., 135: 1669-77 (1996) 1996

      Show Abstract
      8978831 8978831
      Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc.
      Reinhardt, D, et al.
      J. Biol. Chem., 268: 10881-7 (1993) 1993

      Show Abstract
      8496153 8496153

      Data Sheet

      Anti-Nidogen, G2 domain, clone JF6 - Data Sheet

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