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AB951 | Anti-Neuron Specific Enolase Antibody

500 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, R IHC, IH(P), WB Rb Serum Polyclonal Antibody
      Catalogue NumberAB951
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neuron Specific Enolase Antibody
      Alternate Names
      • NSE
      • gamma Enolase
      • Neural Enolase
      • Enolase 2
      Background InformationThe glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha'-enolase), neuron-specific enolase (NSE; gamma,gamma'-enolase, also known as 14-3-2 protein and NSP)(Grasso et al. 1977; Schmechel et al. 1979) and the short-lived heterodimer alpha, gamma'-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa (Schmechel et al. 1978), which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors (Marangos 1985). NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome (DeGiorgio et al. 1995; Yamazaki et al. 1995).
      Product Information
      • Brain tissue
      PresentationRabbit antiSerum. Liquid in 0.1M NaCl. Contains 0.1% sodium azide. Dilute if necessary, with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used).
      ApplicationDetect Neuron Specific Enolase using this Anti-Neuron Specific Enolase Antibody validated for use in IH, IH(P) & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blot: 1:100-1:1,000 (ECL) On denatured Western blots of purified bovine NSE the antibody recognizes a single band at approximately 39 kDa. On Western blots of whole rat brain lysates (non-reduced) a more complex series of bands is observed ranging from 78/80 kDa (NSE homodimer) to 20 kDa (gamma-enolase degradation product).

      Immunohistochemistry: 1:100-1:1,000 (ABC) The antibody has been used successfully on formaldehyde-fixed cryostat, Vibratome and de-paraffinized tissues. For indirect immunofluorescence procedures use at a dilution of 1:80-1:160. Dilute to working concentration with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used).

      Optimal working dilutions must be determined by the end user.


      This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

      1) 3 x 10 minute washes in TBS (without Triton).

      2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody) & Triton (must be optimized, start @ 0.1%).

      3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

      4) 3 x 10 minute washes in TBS.

      5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

      6) 3 x 10 minute washes in TBS.

      7) ABC Elite (1:200 Vector Labs) in TBS.

      8) 2 x 10 minute washes in TBS.

      9) 1 x 10 minute wash in phosphate buffer (no saline).

      10) DAB reaction with 0.06% NiCl added for intensification.

      11) 2 x 10 minute washes in PBS.

      12) 1 x 10 minute wash in phosphate buffer (no saline).
      Biological Information
      ImmunogenSynthetic peptide obtained from hNSE.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAB951 stains many neuronal cell bodies and processes within the central and peripheral nervous system.
      Species Reactivity
      • Bovine
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
      Gene Symbol
      • ENO2
      • NSE
      • Gamma-enolase
      • EC
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
      COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
      SIZE: 434 amino acids; 47269 Da
      SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
      TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
      SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family.
      Molecular Weight~39 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      References | 27 Available | See All References

      Reference overviewPub Med ID
      Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.
      Korzhevskii, DE; Sukhorukova, EG; Kirik, OV; Grigorev, IP
      European journal of histochemistry : EJH 59 2530 2015

      Show Abstract
      26428887 26428887
      Co-Application of Corticosterone and Growth Hormone Upregulates NR2B Protein and Increases the NR2B:NR2A Ratio and Synaptic Transmission in the Hippocampus.
      Mahmoud, GS; Amer, AS
      Sultan Qaboos University medical journal 14 e486-94 2014

      Show Abstract
      25364551 25364551
      NDRG2 as a marker protein for brain astrocytes.
      Flügge, G; Araya-Callis, C; Garea-Rodriguez, E; Stadelmann-Nessler, C; Fuchs, E
      Cell and tissue research 357 31-41 2014

      Show Abstract
      24816982 24816982
      A ROCK Inhibitor Blocks the Inhibitory Effect of Chondroitin Sulfate Proteoglycan on Morphological Changes of Mesenchymal Stromal/Stem Cells into Neuron-Like Cells.
      Lim, HS; Joe, YA
      Biomolecules & therapeutics 21 447-53 2013

      Show Abstract
      24404335 24404335
      Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus.
      Araya-Callís, C; Hiemke, C; Abumaria, N; Flugge, G
      Psychopharmacology 224 209-22 2012

      Show Abstract
      22610521 22610521
      Chotosan (Diaoteng San)-induced improvement of cognitive deficits in senescence-accelerated mouse (SAMP8) involves the amelioration of angiogenic/neurotrophic factors and neuroplasticity systems in the brain.
      Qi Zhao,Takako Yokozawa,Koichi Tsuneyama,Ken Tanaka,Takeshi Miyata,Notoshi Shibahara,Kinzo Matsumoto
      Chinese medicine 6 2011

      Show Abstract Full Text Article
      21943225 21943225
      Nitric oxide and interleukin-1beta mediate noradrenergic induced corticotrophin-releasing hormone release in organotypic cultures of rat paraventricular nucleus.
      CH Hsieh, HY Li, JC Chen
      Neuroscience 165 1191-202 2010

      Show Abstract
      20004705 20004705
      Localized donor cells in brain of a Hunter disease patient after cord blood stem cell transplantation.
      Ken Araya,Norio Sakai,Ikuko Mohri,Kuriko Kagitani-Shimono,Takeshi Okinaga,Yoshiko Hashii,Hideaki Ohta,Itsuko Nakamichi,Katsuyuki Aozasa,Masako Taniike,Keiichi Ozono
      Molecular genetics and metabolism 98 2009

      Show Abstract
      19556155 19556155
      Postnatal changes in the Rexed lamination and markers of nociceptive afferents in the superficial dorsal horn of the rat.
      Louis-Etienne Lorenzo,Michele Ramien,Manon St Louis,Yves De Koninck,Alfredo Ribeiro-da-Silva
      The Journal of comparative neurology 508 2008

      Show Abstract
      18383051 18383051
      Short-term biological safety of a photoelectric dye used as a component of retinal prostheses.
      Kazuo Okamoto,Toshihiko Matsuo,Takayuki Tamaki,Akihito Uji,Hiroshi Ohtsuki
      Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs 11 2008

      Show Abstract
      18414992 18414992

      Data Sheet


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      Life Science Research > Antibodies and Assays > Primary Antibodies