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05-830 | Anti-Neurofilament L Antibody, clone DA2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, H, M, Po, R, Rb WB, IHC M Purified Monoclonal Antibody
      Catalogue Number05-830
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Neurofilament L Antibody, clone DA2
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationThis Anti-Neurofilament L Antibody, clone DA2 is validated for use in WB, IH for the detection of Neurofilament.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenPurified and enzymatically dephosphorylated pig neurofilament, light chain
      Cloneclone DA2
      Specificityneurofilament, light chain
      Species Reactivity
      • Avian
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFL
      • NF68
      • CMT1F
      • NF-L
      • CMT2E
      • NFL
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07196 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 543 amino acids; 61517 Da
      DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
      PTM: O-glycosylated (By similarity).
      DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
      SIMILARITY: Belongs to the intermediate filament family.
      MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
      Molecular Weight68kDa and a protein fragment, 56kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from a rat brain microsomal preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Neurofilament, light chain, clone DA2 - 27008 27008
      Anti-Neurofilament, light chain, clone DA2 -2806395 2806395


      Reference overviewPub Med ID
      Neurofilaments and neurological disease.
      Al-Chalabi, Ammar and Miller, Christopher C J
      Bioessays, 25: 346-55 (2003) 2003

      12655642 12655642
      Does neurofilament phosphorylation regulate axonal transport?
      Shea, Thomas B, et al.
      Trends Neurosci., 26: 397-400 (2003) 2003

      Show Abstract
      12900166 12900166
      Kinesin, dynein and neurofilament transport.
      Shea, T B and Flanagan, L A
      Trends Neurosci., 24: 644-8 (2001) 2001

      Show Abstract
      11672808 11672808
      Neurofilament protein synthesis and phosphorylation.
      Grant, P and Pant, H C
      J. Neurocytol., 29: 843-72 (2000) 2000

      Show Abstract
      11466475 11466475

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      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies