AB1991 Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat

AB1991
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ma, Rp IH(P), WB, IHC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1991
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatSerum
      PresentationNeat rabbit antisera.
      Applications
      ApplicationDetect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat validated for use in IH(P), WB, IH.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:250-1:1,000 AB1991 is not affected by the level of neurofilament phosphorylation and is particularly good for revealing dendritic and perikaryal neurofilaments.

      Immunoblotting

      Electron microscopy

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenE. Coli recombinant fusion protein containing 37 lysine-serine-proline repeats of rat NF-H.
      Epitopelysine-serine-proline repeat
      HostRabbit
      SpecificityStrong reactivity to the major neurofilament subunit HF-H. Since a second neurofilament subunit, NF-M, also contains a few lysine-serine-proline (KSP) sequences, there is generally some reactivity with this protein also. AB1991 stains both phosphorylated and dephosphorylated neurofilaments.
      Species Reactivity
      • Avian
      • Mammals
      • Reptile
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NFH
      • NF-H
      • KIAA0845
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      2RABBIT ANTI-NEUROFILAMENT H - 31671093167109
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 24285192428519
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 24627402462740
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY2984181
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 20703992070399
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 22341352234135
      RABBIT ANTI-NEUROFILAMENT H -25729922572992
      RABBIT ANTI-NEUROFILAMENT H -26109672610967
      RABBIT ANTI-NEUROFILAMENT H -27284232728423
      RABBIT ANTI-NEUROFILAMENT H -27768862776886

      References

      Reference overviewApplicationSpeciesPub Med ID
      An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.
      Aven, L; Paez-Cortez, J; Achey, R; Krishnan, R; Ram-Mohan, S; Cruikshank, WW; Fine, A; Ai, X
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  28  897-907  2014

      Show Abstract
      Western Blotting24221086 24221086
      Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis.
      Shen, C; Lu, Y; Zhang, B; Figueiredo, D; Bean, J; Jung, J; Wu, H; Barik, A; Yin, DM; Xiong, WC; Mei, L
      The Journal of clinical investigation  123  5190-202  2013

      Show Abstract
      24200689 24200689
      Rescue of peripheral and CNS axon defects in mice lacking NMNAT2.
      Gilley, J; Adalbert, R; Yu, G; Coleman, MP
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  13410-24  2013

      Show Abstract
      23946398 23946398
      β-Catenin gain of function in muscles impairs neuromuscular junction formation.
      Wu, H; Lu, Y; Barik, A; Joseph, A; Taketo, MM; Xiong, WC; Mei, L
      Development (Cambridge, England)  139  2392-404  2012

      Show Abstract
      22627288 22627288
      Astrocyte-secreted factors modulate the developmental distribution of inhibitory synapses in nucleus laminaris of the avian auditory brainstem.
      Matthew J Korn,Scott J Koppel,Lan H Li,Divya Mehta,Sonia B Mehta,Armin H Seidl,Karina S Cramer
      The Journal of comparative neurology  520  2012

      Show Abstract
      22020566 22020566
      VEGF-Induced Growth Cone Enhancement Is Diminished by Inhibiting Tyrosine-Residue 1214 of VEGFR-2.
      Daniel Foehring,Beate Brand-Saberi,Carsten Theiss
      Cells, tissues, organs  196  2012

      Show Abstract
      22433970 22433970
      Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.
      Wu, H; Lu, Y; Shen, C; Patel, N; Gan, L; Xiong, WC; Mei, L
      Neuron  75  94-107  2012

      Show Abstract
      Western Blotting22794264 22794264
      Olig1 function is required for remyelination potential of transplanted neural progenitor cells in a model of viral-induced demyelination.
      Whitman, LM; Blanc, CA; Schaumburg, CS; Rowitch, DH; Lane, TE
      Experimental neurology  235  380-7  2012

      Show Abstract
      Immunohistochemistry22449475 22449475
      A method to isolate and culture expand human dental pulp stem cells.
      Stan Gronthos,Agnieszka Arthur,P Mark Bartold,Songtao Shi
      Methods in molecular biology (Clifton, N.J.)  698  2011

      Show Abstract
      21431514 21431514
      Spreading of prions from the immune to the peripheral nervous system: a potential implication of dendritic cells.
      Dorban, Gauthier, et al.
      Histochem. Cell Biol., 133: 493-504 (2010)  2010

      Show Abstract
      20238136 20238136

      Data Sheet

      Title
      RABBIT ANTI-NEUROFILAMENT H

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